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    Micropropagation of Launaea cornuta - an important indigenous vegetable and medicinal plant

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    U16ArtAmbajoMicropropagationNothomDev.pdf (259.2Kb)
    Date
    2016-08-10
    Author
    Ambajo, F.
    Matheka, J.M.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract
    An efficient micropropagation protocol was developed for the medicinal plant Launaea cornuta using green house axillary buds as explants. The best sterility was obtained at 30% (v/v) local bleach (JIK). Maximum shoot induction rate was achieved when axillary buds were cultured on Murashige and Skoog (MS) Media supplemented with 0.5 mg/L of 6-benzylaminopurine (BAP) for 3 weeks. The highest number of shoot multiplication was obtained when induced shoots were culture on MS media supplemented with 0.5 mg/L BAP and 0.2 mg/L NAA for 30 days. The best rooting response with regard to average root length, rooting percentage and number of roots was achieved within 4 weeks of culture of excised shoots on MS media having 0.5 mg/L BAP. Regenerated plants were successfully acclimatized and about 80 to 90% of plantlets survived under ex vitro conditions. About 170 plants were produced from a single nodal bud of L. cornuta after 60 days. A reproducible protocol was established for in vitro propagation of L. cornuta, an important indigenous vegetable with high medicinal value.
    https://dx.doi.org/10.5897/ajb2016.15260
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/1259
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.5897/ajb2016.15260
    Research Themes
    BIOTECH & PLANT BREEDING
    IITA Subjects
    Genetic Improvement; Plant Breeding; Tissue Culture
    Agrovoc Terms
    Tissue Culture; Micropropagation; Medicinal Plant
    Regions
    Africa; East Africa; Southern Africa
    Countries
    Kenya; Malawi; Mozambique; Tanzania; Uganda; Zimbabwe
    Journals
    African Journal of Biotechnology
    Collections
    • Journal and Journal Articles4136
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