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High frequency shoot regeneration of various cultivars of banana (Musa sp.)
Abstract
An efficient, simple, and rapid regeneration system was
established for bananas (Musa sp.) using sections of corm containing intercalary
meristematic tissues as explants. Six different cultivars of banana
(‘Mpologoma’, ‘Nakitembe’, ‘Mbwazirume’, ‘Pisang awak’, ‘Sukali
ndiizi’, and ‘FHIA-17’) with diverse genetic constitution and ploidy levels
were regenerated on Murashige and Skoog (MS) medium supplemented
with 5 mgl−1 of 6-benzylaminopurine. All the cultivars showed relatively
high regeneration efficiency. About 93–97% of the explants regenerated,
producing, on average, 12–13 shoots from whole section and 16–19 shoots
in total from quarter pieces of each section. Regeneration from intercalary
meristematic tissues offers a simple, efficient method for transformation of
a broad range of banana cultivars, including East African Highland
bananas.
KEYWOR
Multi standard citation
Permanent link to this item
https://hdl.handle.net/20.500.12478/2863Digital Object Identifier (DOI)
http://dx.doi.org/10.1080/15427520802123679