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    Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants

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    U18ArtOnyiloSilencingInthomDev.pdf (3.201Mb)
    Date
    2018-03-13
    Author
    Onyilo, F.
    Tusiime, Geoffrey
    Tripathi, J.N.
    Chen, L.H.
    Falk, B.
    Stergiopoulos, I.
    Tushemereirwe, W.K.
    Kubiriba, J.
    Tripathi, L.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract
    Pseudocercospora fijiensis, causal agent of the black Sigatoka disease (BSD) of Musa spp., has spread globally since its discovery in Fiji 1963 to all the banana and plantain growing areas across the globe. It is becoming the most damaging and economically important disease of this crop. The identification and characterization of genes that regulate infection processes and pathogenicity in P. fijiensis will provide important knowledge for the development of disease-resistant cultivars. In many fungal plant pathogens, the Fus3 and Slt2 are reported to be essential for pathogenicity. Fus3 regulates filamentous-invasion pathways including the formation of infection structures, sporulation, virulence, and invasive and filamentous growth, whereas Slt2 is involved in the cell-wall integrity pathway, virulence, invasive growth, and colonization in host tissues. Here, we used RNAi-mediated gene silencing to investigate the role of the Slt2 and Fus3 homologs in P. fijiensis in pathogen invasiveness, growth and pathogenicity. The PfSlt2 and PfFus3 silenced P. fijiensis transformants showed significantly lower gene expression and reduced virulence, invasive growth, and lower biomass in infected leaf tissues of East African Highland Banana (EAHB). This study suggests that Slt2 and Fus3 MAPK signaling pathways play important roles in plant infection and pathogenic growth of fungal pathogens. The silencing of these vital fungal genes through host-induced gene silencing (HIG) could be an alternative strategy for developing transgenic banana and plantain resistant to BSD.
    http://dx.doi.org/10.3389/fpls.2018.00291
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3203
    Non-IITA Authors ORCID
    Leena Tripathihttps://orcid.org/0000-0001-5723-4981
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.3389/fpls.2018.00291
    IITA Subjects
    Banana; Plant Production; Plantain
    Agrovoc Terms
    Pseudocercospora; Protein Kinase; Pathogenicity; Black Sigatoka Disease; Plantains; Bananas; Host Plant
    Regions
    Africa; East Africa
    Journals
    Frontiers in Plant Science
    Collections
    • Journal and Journal Articles4136
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