Welcome to the International Institute of Tropical Agriculture Research Repository
What would you like to view today?
The occurrence of African cassava mosaic virus and East African cassava mosaic Cameroon virus in natural hosts other than cassava in Nigeria
Dixon, Alfred G.O.
MetadataShow full item record
Cassava mosaic disease (CMD) in Africa is caused by six distinct cassava mosaic viruses (family eminiviridae, genus Begomovirus). Among them, African cassava mosaic virus (ACMV) and East African cassava mosaicCameroon virus (EACMCV) have been reported in CMD-infected cassava plants in Nigeria. Based on PCR tests using virus-specific primers, a leguminous plant Senna occidentalis and the weed Combreturn confertumwere previously identified as natural hosts for ACMV and EACMCV and castor oil plant Ricinus cornmunis as a host for ACMV. In a recent study, we identified a second leguminous plant Leucana leucocephala as a host for ACMV and EACMCV. In order to confirm the PCR results, we have amplified 1 kbp DNA fragments specific to ACI and AV2 open reading frames (OW) of ACMV and AC3 and ACI ORFs of EACMCV. The amplicons were cloned and nucleotide sequences compared with corresponding sequences of ACMV and EACMCV isolates from cassava. All ACMV isolates from the four natural hosts showed sequence identity between 95 and 96 percent with cassava isolates. Similarly, all EACMCV isolates from the three natural hosts showed nucleotide sequence identity above 98 percent with isolates from cassava. The results confirm the natural occurrence of ACMV and EACMCV in four plant species other than cassava and indicate that theymay play a role in the epidemiology of CMD in Nigeria.
Permanent link to this itemhttps://hdl.handle.net/20.500.12478/3476
Showing items related by title, author, creator and subject.
Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria Alabi, O.J.; Ogbe, F.O.; Bandyopadhyay, Ranajit; Kumar, P.L.; Dixon, Alfred G.O.; Hughes, Jacqueline d'Arros; Naidu, R.A. (2008)Cassava mosaic disease (CMD) caused by African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) is the major constraint to cassava production in Nigeria. Sequences of the DNA-A component ...
Multiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava Alabi, O.J.; Kumar, P.L.; Naidu, R.A. (2008)A multiplex PCR was developed for simultaneous detection of African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) in cassava affected with cassava mosaic disease (CMD). One set of three ...
First report of the occurrence of African cassava mosaic virus in a mosaic disease of soybean in Nigeria Mgbechi-Ezeri, J.U.; Alabi, O.J.; Naidu, R.A.; Kumar, P.L. (2008)African cassava mosaic virus (ACMV; genus Begomovirus, family Geminiviridae) is one of six viruses documented in cassava (Manihot esculenta Crantz.) plants showing cassava mosaic disease in sub-Saharan Africa (SSA). In ...