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    Development of a semi selective medium for isolation of Xanthomonas campestris pv. musacearum from banana plants

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    Tripathi, BXW semi-selective medium, EJPP, 20071.pdf (229.2Kb)
    Date
    2007
    Author
    Tripathi, L.
    Tripathi, J.N.
    Tushemereirwe, W.K.
    Bandyopadhyay, Ranajit
    Type
    Journal Article
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    Abstract
    Banana Xanthomonas wilt, caused by Xanthomonas campestris pv. musacearum, is a new threat to banana cultivation in eastern Africa. The causal bacterium grows slowly in culture and is easily overgrown by contaminants. A selective culture medium for isolation of X. c. pv. musacearum will facilitate disease study. A medium that suppressed saprophytic growth and possessed diagnostic characters for the pathogen was developed. Various carbon sources were tested with two isolates of X. c. pv. musacearum, and sucrose was selected as main carbon source. The susceptibility of X. c. pv. musacearum and other bacterial strains was tested with 29 different antibiotics. Cephalexin and cycloheximide had no effect on X. c. pv. musacearum but cephalexin inhibited most of the saprophytes and cycloheximide inhibited the fungal contaminants. Based on these studies, we have developed a semi-selective medium YTSA-CC containing yeast extract (1%), tryptone (1%), sucrose (1%), agar (1.5%), cephalexin (50 mg l−1) and cycloheximide (150 mg l−1), pH 7.0. The pathogen X. c. pv. musacearum was easily identified as yellowish, mucoid and circular colonies on YTSA-CC medium. This simple semi-selective medium was effective for isolation of X. c. pv. musacearum from infected banana tissues and soil, and it should be a valuable tool in ecological and epidemiological studies.
    https://doi.org/10.1007/s10658-006-9083-7
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3483
    Digital Object Identifier (DOI)
    https://doi.org/10.1007/s10658-006-9083-7
    IITA Subjects
    Banana; Farm Management; Climate Change; Disease Control; Plant Production; Handling, Transport, Storage And Protection Of Agricultural Products; Plant Diseases; Plant Breeding; Plant Genetic Resources
    Agrovoc Terms
    Antibiotic Susceptibility; Banana Xanthomonas; Wilt Carbon Sources; Semi-Selective Medium; Xanthomonas Campestris Pv. Musacearum; Pathogen; Saprophytic
    Regions
    Africa; East Africa; West Africa
    Countries
    Uganda; Nigeria
    Collections
    • Journal and Journal Articles4127
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