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    Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level

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    Date
    2000
    Author
    Bosque-Pérez, N.A.
    May, G.
    Artzen, C.
    Type
    Journal Article
    Metadata
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    Abstract
    An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains.
    https://doi.org/10.17660/ActaHortic.2000.540.21
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/5326
    Digital Object Identifier (DOI)
    https://doi.org/10.17660/ActaHortic.2000.540.21
    IITA Subjects
    Banana; Food Security; Genetic Improvement; Plant Breeding; Plant Diseases
    Agrovoc Terms
    Genomics; Bananas; Clones; Musa; Agrobacterium
    Regions
    Africa; Acp; West Africa; North America
    Countries
    Nigeria; United States
    Collections
    • Journal and Journal Articles4137
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