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    EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences

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    Date
    2018
    Author
    Olodo, K.F.
    Gueye, M.C.
    Calatayud, C.
    Diop, B.M.
    Kane, N.A.
    Ngom, A.
    Ntui, V.O.
    Barreto, M.M.S.
    Uyoh, E.A.
    Abraham, S.
    Vigouroux, Y.
    Billot, C.
    Barnaud, A.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Digitaria exilis is an important indigenous cereal in West Africa. The first fonio reference transcriptome was released and became a key tool for developing new molecular markers contributing to a better understanding of its genetic diversity. A total of 126 new putative primer pairs were successfully designed in 37,327 unigenes from the D. exilis transcriptome. Thirty-seven primer pairs were randomly selected and tested for their ability to cross-amplify to related species. Clear amplification patterns were observed on 24 primer pairs. Of these, 71, 74 and 35% showed polymorphism in three species: D. exilis, D. longiflora and D. iburua. The transferability from D. exilis was 96% to D. longiflora and 71% to D. iburua. The new SSR markers confirmed the close genetic proximity of D. exilis with D. longiflora and its stronger genetic difference of D. exilis from D. iburua. These markers will be valuable for completing future knowledge on Digitaria evolutionary history, and for testing gene flows between related species.
    http://dx.doi.org/10.1017/s147926211800040
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/5211
    Non-IITA Authors ORCID
    Valentine Ntuihttps://orcid.org/0000-0003-4709-8087
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1017/s147926211800040
    Agrovoc Terms
    Digitaria; Microsatellites; Poaceae; West Africa
    Regions
    Africa; West Africa
    Countries
    Benin; Burkina Faso; Guinea; Mali; Niger; Senegal; Togo
    Journals
    Plant Genetic Resources
    Collections
    • Journal and Journal Articles4839
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