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    CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

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    Journal Article (2.042Mb)
    Date
    2019
    Author
    Tripathi, J.N.
    Ntui, V.O.
    Ron, M.
    Muiruri, S.K.
    Britt, A.
    Tripathi, L.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one B genome, cannot be used as parents for crop improvement. Here, we report a strategy to inactivate the eBSV by editing the virus sequences. The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. Seventy-five percent of the edited events remained asymptomatic in comparison to the non-edited control plants under water stress conditions, confirming inactivation of eBSV into infectious viral particles. This study paves the way for the improvement of B genome germplasm and its use in breeding programs to produce hybrids that can be globally disseminated.
    https://dx.doi.org/10.1038/s42003-019-0288-7
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/6857
    IITA Authors ORCID
    Jaindra Tripathihttps://orcid.org/0000-0002-6366-917X
    Ntuihttps://orcid.org/0000-0003-4709-8087
    Leena Tripathihttps://orcid.org/0000-0001-5723-4981
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1038/s42003-019-0288-7
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Banana; Disease Control; Food Security; Plant Breeding; Plant Diseases; Plant Health; Plant Production; Plantain
    Agrovoc Terms
    Bananas; Musa; Dna; Breeding; Viruses; Plantains; Genomes
    Regions
    Africa; East Africa
    Countries
    Uganda
    Hubs
    Eastern Africa Hub
    Journals
    Communications Biology
    Collections
    • Journal and Journal Articles4835
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