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Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt

dc.contributor.authorNakato, G.V.
dc.contributor.authorAkinbade, S.A.
dc.contributor.authorKumar, P.L.
dc.contributor.authorBandyopadhyay, Ranajit
dc.contributor.authorBeed, Fen D.
dc.date.accessioned2019-12-04T11:08:58Z
dc.date.available2019-12-04T11:08:58Z
dc.date.issued2013-12
dc.identifier.citationNakato, G.V., Akinbada, S.A., Kumar, P.L., Bandyopadhyay, R. & Beed, F. (2013). Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt. In G. Blomme, P. van Asten and B. Vanlauwe, Banana systems in the humid highlands of sub-Saharan Africa. (p. 93-100). Wallingford: CABI.
dc.identifier.isbn978-1-78064-231-4
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1948
dc.description.abstractRabbit polyclonal antibodies (PAbs) were produced against a pure isolate of Xanthomonas campestris pv. musacearum (Xcm), which is the causal organism of banana Xanthomonas wilt (BXW) disease of banana (Musa spp.) in the Great Lakes region of Africa. Anti-Xcm PAbs were used to develop a direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA) for the detection of Xcm in leaf and pseudostem tissues of infected banana and plantain (Musa spp.). The anti-Xcm PAbs in DAC-ELISA specifically reacted with Xcm but not with Escherichia coli, X. vasicola pv. vasculorum of maize and sugarcane or X. vasicola pv. holcicola of sorghum. Results of the DAC-ELISA detection of Xcm are comparable with the results of PCR and culture-based detection assays for the same pathogen.
dc.description.sponsorshipAssociation for Strengthening Agricultural Research in Eastern and Central Africa
dc.format.extent93-100
dc.language.isoen
dc.publisherCentre for Agriculture and Bioscience International
dc.subjectPolyclonal Antibodies
dc.subjectBananas
dc.subjectDiseases
dc.subjectRabbit Polyclonal Antibodies
dc.subjectBanana Xanthomonas Wilt
dc.subjectXanthomonas Campestris Pv. Musacearum
dc.titleDevelopment of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt
dc.typeBook Chapter
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionCentral Africa
cg.coverage.regionEast Africa
cg.coverage.countryBurundi
cg.coverage.countryCongo, Dr
cg.coverage.countryKenya
cg.coverage.countryRwanda
cg.coverage.countryTanzania
cg.coverage.countryUganda
cg.authorship.typesCGIAR single centre
cg.iitasubjectBanana
cg.iitasubjectPlant Diseases
cg.howpublishedFormally Published
cg.publicationplaceWallingford, United Kingdom
cg.accessibilitystatusLimited Access
local.dspaceid85224
cg.targetaudienceScientists


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