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dc.contributor.authorRamathani, I.
dc.contributor.authorBeed, Fen D.
dc.date.accessioned2019-12-04T11:08:59Z
dc.date.available2019-12-04T11:08:59Z
dc.date.issued2013-12
dc.identifier.citationRamathani, I. & Beed, F. (2013). Use of DNA capture kits to collect Xanthomonas campestris pv. musacearum and banana bunchy top virus pathogen DNA for molecular diagnostics. In G. Blomme, P. van Asten and B. Vanlauwe, Banana systems in the humid highlands of sub-Saharan Africa (p. 109-115). Wallingford: CABI.
dc.identifier.isbn978-1-78064-231-4
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1952
dc.description.abstractBanana Xanthomonas wilt, caused by the bacterium Xanthomonas campestris pv. musacearum, and banana bunchy top virus disease (BBTD), caused by the banana bunchy top virus (BBTV), are two of the most devastating diseases of banana in East and Central Africa, and cause significant losses in food security and income for millions of farmers, traders and consumers. To prevent the establishment and spread of the diseases across the region, control interventions must be rapidly deployed. A first and critical step towards deployment of appropriate disease management strategies is the rapid and precise diagnosis of the causal agent of disease. The diagnosis of Xanthomonas wilt in the field based on disease symptoms is often confused with another disease that causes wilting that is due to the fungus Fusarium oxysporum f.sp. cubense, so diagnostic tools that are easy to use, cost-effective and reliable are required to help field workers. BBTD symptoms can also be confused with nutrient deficiencies or varietal differences. To this end, three types of portable kits were evaluated for their performance in capturing pathogen DNA from the field for use in precise, molecular-based pathogen diagnostics under controlled laboratory conditions. All three prototypes tested – FTA cards, the PhytoPASS kit and two-minute DNA extraction dipsticks – gave excellent results and methods were optimized for sample collection in the field, DNA extraction and PCRbased diagnostics for X. campestris pv. musacearum and BBTV. The benefits of using DNA capture kits included: collection of pathogen DNA in a cheap and practical manner, safe and fast transfer of high integrity pathogen DNA across country borders, rapid and precise diagnosis using state-of-the-art molecular technologies and direct comparison of results from geographically diverse samples. This is possible because analysis is performed in a uniform manner in terms of method, date, equipment, reagents and technical staff. Technical support was provided to workers in the field to help them differentiate symptoms based on diagnostic results from laboratory analyses; such support could also be provided to regulatory officials at borders to ensure that, for example, planting material is free from disease. Countries involved in testing the DNA capture kits for X. campestris pv. musacearum included Burundi, Democratic Republic of Congo (DR Congo), Kenya, Rwanda, Tanzania and Uganda. The same countries, with Zambia also included, were also tested for BBTV. While no positive results for X. campestris pv. musacearum were obtained from samples originating from surveys in Burundi, subsequent DNA capture kits from samples from Bubanza and Cankuzo provinces did test positive, thus confirming an outbreak of Xanthomonas wilt in this country. BBTV was confirmed in DR Congo, Burundi, Rwanda and Zambia.
dc.description.sponsorshipAssociation for Strengthening Agricultural Research in Eastern and Central Africa
dc.description.sponsorshipFood and Agriculture Organization of the United Nations
dc.format.extent109-115
dc.language.isoen
dc.publisherCentre for Agriculture and Bioscience International
dc.subjectDna
dc.subjectBanana Bunchy Top Virus
dc.subjectBananas
dc.subjectFood Security
dc.subjectXanthomonas Campestris Pv. Musacearum
dc.subjectEast And Central Africa
dc.titleUse of DNA capture kits to collect Xanthomonas campestris pv. musacearum and banana bunchy top virus pathogen DNA for molecular diagnostics
dc.typeBook Chapter
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionCentral Africa
cg.coverage.regionEast Africa
cg.coverage.regionSouthern Africa
cg.coverage.countryBurundi
cg.coverage.countryCongo, Dr
cg.coverage.countryKenya
cg.coverage.countryRwanda
cg.coverage.countryTanzania
cg.coverage.countryUganda
cg.coverage.countryZambia
cg.authorship.typesCGIAR single centre
cg.iitasubjectBanana
cg.iitasubjectDisease Control
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Genetic Resources
cg.howpublishedFormally Published
cg.publicationplaceWallingford, UK
cg.accessibilitystatusLimited Access
local.dspaceid85249
cg.targetaudienceScientists


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