• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Books and Book Chapters
    • Books and Book Chapters
    • View Item
    •   Home
    • Books and Book Chapters
    • Books and Book Chapters
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Oppotunities for biotechnology in cowpea

    Thumbnail
    View/Open
    U97InbkMontiOpportunitiesNothomNodev.pdf (2.743Mb)
    Date
    1997
    Author
    Monti, L.
    Murdock, L.
    Thottappilly, G.
    Type
    Book Chapter
    Metadata
    Show full item record
    Abstract/Description
    Several applications of biotechnology have been successfully used recently in cowpea. A molecular map of cowpea has been constructed using RFLP markers, and this has already facilitated the localization of certain quantitative trait loci; gene chromosome localization using in situ hybridization is in progress. Appropriate bioassays have been developed that have facilitated the identification of candidate genes for insect pest resistance in cowpea, including Bacillus rhttrins>ensis protoxin genes, and genes coding a-amylase inhibitor, protease inhibitor, and lectins. Since cowpeas are "recalcitrant" to regenerate "in vitro", several attempts have been made to develop a reliable protocol for differentiating shoots from calli obtained through in vitro tissue cultures. Thus far, only regeneration from already meristem-rich tissues has been obtained. The best results were obtained using the herbicide, thidiazuron. As a growth regulator to induce multiple bud proliferation. Agrobdctcriiuu-medrdled plant genetic transformation remains an approach that requires considerable further work to be efficient. Direct plasmid DNA transfer into meristemalic cells has also been attempted using micro projectile bombardment; rates of genetic transformation are too low to be useful. Recently, two new transformation methods were set up on in vivo plants: the first is based on electro injection of plasmid DNA directly into meristemalic cells, and the second involves the inoculation of buds with Agrobacterium; these two methods do not need in vitro regeneration and are giving promising results.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3990
    IITA Subjects
    Cowpea; Livelihoods; Pests Of Plants; Plant Diseases; Plant Health; Genetic Improvement; Plant Genetic Resources; Post-Harvesting Technology; Plant Breeding; Plant Production; Disease Control
    Agrovoc Terms
    Meristemalic Cells; Agrobacterium; Transformation; Genetic; Pests; Biotechnology; Cowpea Genome
    Regions
    Acp; Africa; Europe; North America; West Africa
    Countries
    Italy; United States; Nigeria
    Collections
    • Books and Book Chapters953
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository