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dc.contributor.authorOkeola, O.G.
dc.contributor.authorMachuka, J.
dc.contributor.authorFasidi, I.O.
dc.identifier.citationOkeola, O.G., Machuka, J. & Fasidi, I.O. (2002). Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod-sucking bug. In C.A. Fatokun, S.A. Tarawali, B.B. Singh, P.M. Kormawa and M. Tamo, Challenges and opportunities for enhancing sustainable cowpea production. Proceedings of the World Cowpea Conference III held at the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria, 4–8 September 2000. Ibadan, Nigeria: IITA. (p. 223-230).
dc.description.abstractThe cowpea beetle, Callosobruchus maculatus, and pod-sucking bug, Clavigralla tomentosicollis, are two of the major insect pests of cowpea in Africa. A lectin was purified from the seeds of the African yam bean (AYB), Sphenostylis stenocarpa, by affinity chromatography on Galactosc-Sepharose 4B. The purified AYB lectin (AYBL) was tested on the two insect pests of cowpea. When C. maculatus larvae were fed on artificial cowpea seed containing 0.2, 2, and 5% (w/w) of dietary lectin, larval mortality ranged from 30 to 88% and delay in number of days to first emer gence from 4-13 days. When AYBL was tested on C. tomentosicollis, nymphal mortalities ranged from 76 to 81% at 1% and 87 to 94% at 2%. From 4 to 8%, no nymph survived up to six days after infestation. The results of these insect bioassays provided a scientific basis for isolating a lectin gene from AYB for the transformation of cowpea.
dc.titleInsecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug
dc.typeConference Paper
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Ibadan
cg.coverage.regionWest Africa
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPests Of Plants
cg.iitasubjectGenetic Improvement
cg.accessibilitystatusLimited Access

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