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    Sample preservation and plant sex prediction in white guinea yam (Dioscorea rotundata Poir.)

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    Journal Article (567.1Kb)
    Date
    2020
    Author
    Agre, P.
    Nwachukwu, C.
    Olasanmi, B.
    Obidiegwu, J.
    Nwachukwu, E.
    Adebola, P.
    De Koeyer, D.
    Asrat, A.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Introduction: Yam (Dioscorea spp.) is an economically important staple food in tropical regions, especially for people in West Africa. Understanding of the flowering behavior of the crop to determine potential manipulation available to accomplish crop improvement at early stage remain key challenge in the yam breeding. The methods that reliably yield quality DNA and distinguishing sex type at the early stage of growth have been a challenge in yam genetics and breeding studies. This study assessed the effect of sample preservation methods on DNA quantity and quality during extraction and potential of DNA marker to diagnose plant sex at the early seedling stage in white Guinea yam. Materials and Methods: Five sample preservation methods were assessed for quality DNA extraction during field leaf tissue collection, namely liquid nitrogen, dry ice, silica gel, 95% ethanol, and oven drying. The predicted sex at the seedling stage using the molecular marker was further validated with the visual score for the sex phenotype at the flowering stage. Results: According to the findings of the present study, the DNA extracted from leaf samples preserved in liquid nitrogen, silica gel, dry ice, and oven drying methods were higher in molecular weights than samples stored in ethanol solution. Yam plant sex diagnosis with the DNA marker (sp16) identified a higher proportion of ZW genotypes (female or monoecious phenotypes) than the ZZ genotypes (male phenotypes) in the studied materials with 74% prediction accuracy. Conclusions: The results from this study provided valuable insights on suitable sample preservation methods for quality DNA extraction and the potential of DNA marker sp16 to predict sex in white Guinea yam.
    https://dx.doi.org/10.30491/jabr.2020.224143.1201
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7145
    IITA Authors ORCID
    Paterne AGREhttps://orcid.org/0000-0003-1231-2530
    Patrick Adebolahttps://orcid.org/0000-0002-5155-6194
    David De Koeyerhttps://orcid.org/0000-0001-8064-6538
    Asrat Asfawhttps://orcid.org/0000-0002-4859-0631
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.30491/jabr.2020.224143.1201
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Plant Breeding; Plant Production; Yam
    Agrovoc Terms
    Dna; Flowering; Preservations; Sex; Genotypes; Phenotypes; Yams
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Journal of Applied Biotechnology Reports
    Collections
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