• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction

    Thumbnail
    View/Open
    S13ArtSharmaImprovedNothomNodev.pdf (354.6Kb)
    Date
    2013-04
    Author
    Sharma, K.
    Bhattacharjee, R.
    Sartie, A.M.
    Kumar, P.L.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or seeds, is not uncommon. The extraction of DNA from different tissue sources often requires different protocols. In this study, a simple protocol was established for the extraction of DNA from leaves, tubers, stems, seeds and even fungal mycelia. The protocol is simple and suitable for high-throughput DNA extraction using automated tissue grinders. It yielded large quantities of DNA (0.4 ?g to 2 mg DNA from 100 mg tissue) of high quality from seeds of maize, soybean, and cowpea, tubers of yam, tuberous roots of cassava, and leaf tissues of banana and plantain, yam, cassava, maize, okra, mango, and other species. DNA was successfully used for the detection of fungal and viral pathogens and the genotyping of yam and cassava by PCR.
    https://dx.doi.org/10.5897/AJB12.2096
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/1192
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.5897/AJB12.2096
    IITA Subjects
    Plant Genetic Resources; Genetic Improvement
    Agrovoc Terms
    Plant Tissues; Pathogens; Genotypes; Dna; Pathogens
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    African Journal of Biotechnology
    Collections
    • Journal and Journal Articles4836
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository