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Genetic architecture of delayed senescence, biomass, and grain yield under drought stress in cowpea

dc.contributor.authorMuchero, W.
dc.contributor.authorRoberts, P.A.
dc.contributor.authorDiop, N.
dc.contributor.authorDrabo, I.
dc.contributor.authorCisse, N.
dc.contributor.authorClose, T.J.
dc.contributor.authorMuranaka, S.
dc.contributor.authorBoukar, O.
dc.contributor.authorEhlers, J.D.
dc.date.accessioned2019-12-04T11:03:42Z
dc.date.available2019-12-04T11:03:42Z
dc.date.issued2013
dc.identifier.citationMuchero, W., Roberts, P.A., Diop, N., Drabo, I., Cisse, N., Close, T.J., ... & Ehlers, J.D. (2013). Genetic architecture of delayed senescence, biomass, and grain yield under drought stress in cowpea. PloS one 8(7):e70041.
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1202
dc.descriptionCowpea
dc.description.abstractThe stay-green phenomenon is a key plant trait with wide usage in managing crop production under limited water conditions. This trait enhances delayed senescence, biomass, and grain yield under drought stress. In this study we sought to identify QTLs in cowpea (Vigna unguiculata) consistent across experiments conducted in Burkina Faso, Nigeria, Senegal, and the United States of America under limited water conditions. A panel of 383 diverse cowpea accessions and a recombinant inbred line population (RIL) were SNP genotyped using an Illumina 1536 GoldenGate assay. Phenotypic data from thirteen experiments conducted across the four countries were used to identify SNP-trait associations based on linkage disequilibrium association mapping, with bi-parental QTL mapping as a complementary strategy. We identified seven loci, five of which exhibited evidence suggesting pleiotropic effects (stay-green) between delayed senescence, biomass, and grain yield. Further, we provide evidence suggesting the existence of positive pleiotropy in cowpea based on positively correlated mean phenotypic values (0.34, r ,0.87) and allele effects (0.07, r ,0.86) for delayed senescence and grain yield across three African environments. Three of the five putative stay-green QTLs, Dro-1, 3, and 7 were identified in both RILs and diverse germplasm with resolutions of 3.2 cM or less for each of the three loci, suggesting that these may be valuable targets for marker-assisted breeding in cowpea. Also, the co-location of early vegetative delayed senescence with biomass and grain yield QTLs suggests the possibility of using delayed senescence at the seedling stage as a rapid screening tool for post-flowering drought tolerance in cowpea breeding. BLAST analysis using EST sequences harboring SNPs with the highest associations provided a genomic context for loci identified in this study in closely related common bean (Phaseolus vulgaris) and soybean (Glycine max) reference genomes.
dc.format.extent1-10.
dc.language.isoen
dc.subjectPlant Production
dc.subjectCowpeas
dc.subjectGenotypes
dc.subjectDrought Stress
dc.subjectBiomass
dc.titleGenetic architecture of delayed senescence, biomass, and grain yield under drought stress in cowpea
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpGrain Legumes
cg.contributor.affiliationUniversity of California
cg.contributor.affiliationInstitut de l'Environnement et de Recherches Agricoles, Burkina Faso
cg.contributor.affiliationInstitut Sénégalais de Recherches Agricoles
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countrySenegal
cg.coverage.countryBurkina Faso
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCowpea
cg.journalPloS one
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid78450
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1371/journal.pone.0070041


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