| dc.contributor.author | Neondo, J.O. |
| dc.contributor.author | Alakonya, A.E. |
| dc.contributor.author | Matheka, J. |
| dc.contributor.author | Masanga, J.O. |
| dc.contributor.author | Kasili, R.W. |
| dc.date.accessioned | 2019-12-04T11:03:49Z |
| dc.date.available | 2019-12-04T11:03:49Z |
| dc.date.issued | 2016-08-03 |
| dc.identifier.citation | Neondo, J.O., Alakonya, A.E., Matheka, J., Masanga, J.O. & Kasili, R.W. (2016). Transformation and regeneration protocol for two farmer preferred open pollinated tropical maize (Zea mays) varieties. Journal of Plant Sciences, 4(4), 80-87. |
| dc.identifier.issn | 2331-0723 |
| dc.identifier.uri | https://hdl.handle.net/20.500.12478/1248 |
| dc.description | Article Purchased |
| dc.description.abstract | Abstract: In vitro regeneration of open pollinated varieties (OPVs) Kakamega Striga Tolerant Population 94 (KSTP’94) and ‘Namba Nane’ alongside a tropical inbred line (CML144) was evaluated using immature zygotic embryos as explants. Four callus induction media (CIM) regimes; Murashige and Skoog (MS), Linsmaier and Skoog (LS), Chu (N6) and N6*(N6
medium fortified with 0.35 gL-1 L-proline and 0.8 mgL-1 AgNO3) were evaluated for their potential to induce callus in the three genotypes. All the media were supplemented with sucrose and five levels of 2, 4-Dichlorophenoxyacetic acid (2, 4-D) (0.5, 1.0, 1.5, 2.0 and 2.5 mgL-1). Resulting calli were matured on MS and N6 basal media supplemented with 60 g/L sucrose and similar concentration levels (0.5, 1.0, 1.5, 2.0 and 2.5 mgL-1) of 2, 4-D while the subsequent embryogenic calli were regenerated on hormone-free media. Transformability of these varieties was assessed via histochemical analysis of β-glucuronidase (GUS) reporter gene following Agrobacterium-mediated transformation. Statistical analyses were done using
Statistical Analysis Software (SAS) and Graphpad Prism softwares with mean separations achieved at 95% confidence intervals. Of the 2 OPVs, KSTP’94 recorded the highest callus induction frequency (84.4%) while Namba Nane (45.6%) had the lowest. Similarly, KSTP, 94 had the highest mean of mature somatic embryos (59.7%) while Namba Nane recorded the
lowest (16.4%). Assessment of regeneration frequencies from embryogenic calli revealed no significant differences among the
3 lines although CML 144 had the highest mean number of juvenile plantlets (36.7%). Analysis of transformation frequency
(upon selection of calli on media with basta) showed that Namba Nane recorded the lowest transformation frequency (average 13.5%) some words missing. Transformation frequency (based on GUS positive calli) of these varieties ranged from 0.8 to 2.1%. This work therefore provides an empirical platform for potential introduction of useful genes into these varieties. |
| dc.format.extent | 80-87 |
| dc.language.iso | en |
| dc.subject | Embryogenesis |
| dc.subject | Maize |
| dc.subject | Regeneration |
| dc.subject | Open Pollination |
| dc.subject | Callus |
| dc.title | Transformation and Regeneration Protocol for Two Farmer Preferred Open Pollinated Tropical Maize (Zea Mays) Varieties |
| dc.type | Journal Article |
| dc.description.version | Peer Review |
| cg.contributor.crp | Maize |
| cg.contributor.affiliation | Jomo Kenyatta University of Agriculture and Technology |
| cg.contributor.affiliation | International Institute of Tropical Agriculture |
| cg.contributor.affiliation | Kenyatta University |
| cg.coverage.region | Africa |
| cg.coverage.region | East Africa |
| cg.coverage.country | Kenya |
| cg.identifier.url | http://www.sciencepublishinggroup.com/j/jps |
| cg.isijournal | ISI Journal |
| cg.authorship.types | CGIAR and developing country institute |
| cg.iitasubject | Maize |
| cg.journal | Journal of Plant Sciences |
| cg.howpublished | Formally Published |
| cg.accessibilitystatus | Open Access |
| local.dspaceid | 78518 |
| cg.targetaudience | Scientists |
| cg.identifier.doi | https://dx.doi.org/10.11648/j.jps.20160404.14 |
