| dc.contributor.author | Ambajo, F. |
| dc.contributor.author | Matheka, J.M. |
| dc.date.accessioned | 2019-12-04T11:03:51Z |
| dc.date.available | 2019-12-04T11:03:51Z |
| dc.date.issued | 2016-08-10 |
| dc.identifier.citation | Ambajo, F. & Matheka, J.M. (2016). Micropropagation of Launaea cornuta-an important indigenous vegetable and medicinal plant. African Journal of Biotechnology, 15(32), 1726-1730. |
| dc.identifier.issn | 1684-5315 |
| dc.identifier.uri | https://hdl.handle.net/20.500.12478/1259 |
| dc.description.abstract | An efficient micropropagation protocol was developed for the medicinal plant Launaea cornuta using green house axillary buds as explants. The best sterility was obtained at 30% (v/v) local bleach (JIK). Maximum shoot induction rate was achieved when axillary buds were cultured on Murashige and Skoog (MS) Media supplemented with 0.5 mg/L of 6-benzylaminopurine (BAP) for 3 weeks. The highest number of shoot multiplication was obtained when induced shoots were culture on MS media supplemented with 0.5 mg/L BAP and 0.2 mg/L NAA for 30 days. The best rooting response with regard to average root
length, rooting percentage and number of roots was achieved within 4 weeks of culture of excised shoots on MS media having 0.5 mg/L BAP. Regenerated plants were successfully acclimatized and about 80 to 90% of plantlets survived under ex vitro conditions. About 170 plants were produced from a single nodal bud of L. cornuta after 60 days. A reproducible protocol was established for in vitro propagation of L. cornuta, an important indigenous vegetable with high medicinal value. |
| dc.format.extent | 1726-1730 |
| dc.language.iso | en |
| dc.subject | Tissue Culture |
| dc.subject | Micropropagation |
| dc.subject | Medicinal Plant |
| dc.title | Micropropagation of Launaea cornuta - an important indigenous vegetable and medicinal plant |
| dc.type | Journal Article |
| dc.description.version | Peer Review |
| cg.contributor.crp | Genebanks |
| cg.contributor.affiliation | Eldoret University |
| cg.contributor.affiliation | International Institute of Tropical Agriculture |
| cg.coverage.region | Africa |
| cg.coverage.region | East Africa |
| cg.coverage.region | Southern Africa |
| cg.coverage.country | Kenya |
| cg.coverage.country | Malawi |
| cg.coverage.country | Mozambique |
| cg.coverage.country | Tanzania |
| cg.coverage.country | Uganda |
| cg.coverage.country | Zimbabwe |
| cg.researchtheme | BIOTECH & PLANT BREEDING |
| cg.identifier.url | http://www.academicjournals.org/AJB |
| cg.authorship.types | CGIAR and developing country institute |
| cg.iitasubject | Genetic Improvement |
| cg.iitasubject | Plant Breeding |
| cg.iitasubject | Tissue Culture |
| cg.journal | African Journal of Biotechnology |
| cg.howpublished | Formally Published |
| cg.accessibilitystatus | Open Access |
| local.dspaceid | 78580 |
| cg.targetaudience | Scientists |
| cg.identifier.doi | https://dx.doi.org/10.5897/ajb2016.15260 |
