• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Cryopreservation for the in perpetuity conservation of yam and cassava genetic resources

    Thumbnail
    View/Open
    S13ArtDumetCryopreservationInthomNodev.pdf (687.8Kb)
    Date
    2013
    Author
    Dumet, D.
    Diebiru, E.
    Adeyemi, A.
    Akinyemi, O.
    Gueye, B.
    Franco, J.
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract/Description
    Cryopreservation via droplet vitrification showed high efficiency for cassava meristems (79% average recovery) when these were excised from in vitro seedlings. The efficiency of the process dropped considerably (to >23%) when meristems were excised from field-grown plants, thus precluding the use of such explants for routine cryobanking. In yam, large disparities were observed in the ability of meristems to produce a shoot after cryopreservation ranging from 0 to 60%, depending on the accession. Overall, better recovery was observed for Dioscorea rotundata than for D. alata, the two main species tested. Using a probabilistic decision support tool and taking into consideration our cryoprocessing capacity, we conclude that processing 100 meristems per accession and retrieving 30 to estimate the recovery rate of the batch are a good compromise for the cryobanking routine.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/1269
    IITA Subjects
    Cassava; Yam
    Agrovoc Terms
    Manihot Esculenta; Meristems; Dioscorea Alata
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    CryoLetters
    Collections
    • Journal and Journal Articles4835
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository