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dc.contributor.authorBerg, E. van den
dc.contributor.authorTiedt, L.R.
dc.contributor.authorCoyne, D.L.
dc.contributor.authorPloeg, A.T.
dc.contributor.authorNavas-Cortes, J.A.
dc.contributor.authorRoberts, P.A.
dc.contributor.authorYeates, G.W.
dc.contributor.authorSubbotin, S.A.
dc.date.accessioned2019-12-04T11:03:56Z
dc.date.available2019-12-04T11:03:56Z
dc.date.issued2013
dc.identifier.citationVan Den Berg, E.,Tiedt, L.R.,Coyne, D.,Ploeg, A.T.,Navas-Cortes, J.A., ... Subbotin, S.A.(2013). Morphological and molecular characterisation and diagnostics of some species of Scutellonema Andrássy, 1958 (Tylenchida: Hoplolaimidae) with a molecular phylogeny of the genus. Nematology, 15(6), 719-745.
dc.identifier.issn1388-5585
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1290
dc.description.abstractScutellonema spp. are widely distributed across tropical and subtropical regions of the world and are associated with numerous agricultural and horticultural crops. Identification of many Scutellonema species is not always reliable, in part because many species share very similar diagnostic characters. In this study, we provide morphological and molecular characterisation of S.brachyurus from the USA and South Africa, S. bradys from Nigeria and three unidentified species from California, USA, New Zealand and Burkina Faso. Morphological descriptions, measurements, light and scanning electron microscopic photos and drawings are given for S. brachyurus. Females of S. brachyurus from the USA (type A) and South Africa (type B) showed a significant variation in the number of sectors and blocks on the lip annuli, ranging from about 4-12 and from 8-20, respectively. Molecular analysis using the D2-D3 of 28S rRNA, ITS rRNA and the COI mtDNA gene sequences revealed two distinct genotypes within S. brachyurus samples: type A (samples from USA, Italy, Korea, Taiwan) and type B (South Africa). Multivariate analyses determined that S. brachyurus from the USA and Taiwan (type A) differed from that from South Africa (type B) mainly in body, tail and DGO lengths, and ratios b , c , c and V. Phylogenetic relationships within Scutellonema are given as inferred from the analyses of the D2-D3 of 28S rRNA, ITS rRNA and the COI mtDNA gene sequences. PCR-RFLP diagnostic profiles and PCR with species-specific primers are developed for the studied Scutellonema species.
dc.format.extent719-745
dc.language.isoen
dc.subjectMorphology
dc.subjectMultivariate Analysis
dc.subjectScutellonema Brachyurum
dc.subjectScutellonema Bradys
dc.subjectTaxonomy
dc.titleMorphological and molecular characterisation and diagnostics of some species of Scutellonema Andrassy, 1958 (Tylenchida: Hoplolaimidae) with a molecular phylogeny of the genus
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationAgricultural Research Council, South Africa
cg.contributor.affiliationNorth-West University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of California
cg.contributor.affiliationInstitute for Sustainable Agriculture, New Zealand
cg.contributor.affiliationPlant Pest Diagnostic Center, California
cg.contributor.affiliationRussian Academy of Sciences
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPest Of Plants
cg.journalNematology
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid78693
cg.identifier.doihttps://dx.doi.org/10.1163/15685411-00002714


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