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dc.contributor.authorAbarshi, M.M.
dc.contributor.authorMohammed, I.
dc.contributor.authorJeremiah, S.C.
dc.contributor.authorLegg, J.P.
dc.contributor.authorKumar, P.L.
dc.contributor.authorHillocks, R.J.
dc.contributor.authorMaruthi, M.N.
dc.date.accessioned2019-12-04T11:04:16Z
dc.date.available2019-12-04T11:04:16Z
dc.date.issued2012-01
dc.identifier.citationAbarshi, M.M., Mohammed, I., Jeremiah, S.C., Legg, J., Kumar, P.L., Hillocks, R.J. & Maruthi, M.N. (2012). Multiplex RT-PCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa. Journal of Virological Methods, 179(1), 176-184.
dc.identifier.issn0166-0934
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1382
dc.description.abstractUniplex and multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocols were developed for the detection of cassava brown streak viruses (CBSVs) in single and mixed infections with cassava mosaic begomoviruses (CMBs) in a tropical crop plant, cassava (Manihot esculenta). CMBs contain ssDNA as their genome (genus Begomovirus, family Geminiviridae) while CBSVs are made up of positive sense ssRNA (genus Ipomovirus, family Potyviridae), and they cause the economically important cassava mosaic and cassava brown streak diseases, respectively, in sub-Saharan Africa. Diagnostic methodologies have long been available for CMBs but they are limited for CBSVs especially in mixed infections. In this study, the two CBSVs, Cassava brown streak virus (CBSV) and Cassava brown streak Uganda virus (CBSUV) occurring singly or in mixed infection with CMBs, African cassava mosaic virus and East African cassava mosaic virus were detected in a single RT-PCR using both previously described and newly designed virus-specific primers. These protocols were highly efficient for detecting CBSVs compared to the existing methods and have great potential to minimize sample handling and contamination. As well as improving the diagnosis of cassava viruses, the development of multiplex RT-PCR protocols have revealed the common occurrence of mixed infections by CBSV and CBSUV in cassava fields of Tanzania and Kenya, which was contrary to the common belief until recently that these two viruses have existed separately. These protocols have implications for diagnosis and epidemiological studies on cassava virus diseases in Eastern Africa.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent176–184
dc.language.isoen
dc.subjectCassava
dc.subjectRna
dc.subjectDna
dc.titleMultiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationUniversity of Greenwich
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.regionSouthern Africa
cg.coverage.countryUganda
cg.coverage.countryKenya
cg.coverage.countryZambia
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCassava
cg.iitasubjectPlant Diseases
cg.journalJournal of Virological Methods
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid79523
cg.identifier.doihttps://dx.doi.org/10.1016/j.jviromet.2011.10.020


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