• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Efficient regeneration and transformation of plantain cv. Gonja manjaya" (Musa spp. AAB) using embryogenic cell suspensions"

    Thumbnail
    View/Open
    S12ArtTripathiEfficientInthomNodev.pdf (743.7Kb)
    Date
    2012-01
    Author
    Tripathi, J.N.
    Muwonge, A.
    Tripathi, L.
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract/Description
    In banana and plantain research, it is essential to establish embryogenic cell suspensions together with a highly efficient regeneration and transformation system. This article describes the development of an embryogenic cell suspension (ECS), regeneration, and transformation for plantain cv. “Gonja manjaya”. ECS was established using highly proliferative multiple buds. The frequency of embryogenic friable callus formation was 56.8% of the cultured explants. Friable embryogenic calli with many translucent proembryos were transferred to liquid medium and homogenous cell suspensions were established within 3–4 mo. Approximately 25,000 to 30,000 plants per 1.0 ml of settled cell volume were regenerated in approximately 13–14 mo. ECSs were transformed using Agrobacterium strain EHA 105 harboring the binary vector pBI121. About 50–60 transgenic plants per 0.5 ml settled cell volume were regenerated on selective medium containing 100 mg l−1 kanamycin. Histochemical GUS assays using different tissues of putatively transformed plants demonstrated stable expression of uidA gene. The presence and integration of the uidA gene were confirmed by PCR and Southern blot analysis, respectively. This is the first report showing establishment of embryogenic cell suspension cultures and Agrobacterium-mediated transformation of an important plantain cultivar, “Gonja manjaya”. This study shows the huge potential for genetic transformation of plantains for disease or pest resistance, as well as tolerance to abiotic factors such as drought stress using this robust regeneration and transformation protocol.
    https://dx.doi.org/10.1007/s11627-011-9422-z
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/1384
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1007/s11627-011-9422-z
    IITA Subjects
    Banana; Plantain
    Agrovoc Terms
    Plantains; Regeneration; Transformation
    Regions
    Africa; East Africa
    Countries
    Uganda
    Journals
    In Vitro Cellular & Developmental Biology - Plant
    Collections
    • Journal and Journal Articles4842
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository