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An ESTderived SNP and SSR genetic linkage map of cassava (Manihot esculenta Crantz)

dc.contributor.authorRabbi, Ismail Y
dc.contributor.authorKulembeka, H.P.
dc.contributor.authorMasumba, E.
dc.contributor.authorMarri, P.
dc.contributor.authorFerguson, M.
dc.date.accessioned2019-12-04T11:04:22Z
dc.date.available2019-12-04T11:04:22Z
dc.date.issued2012
dc.identifier.citationRabbi, I.Y., Kulembeka, H., Masumba, E., Marri, P. & Ferguson, M. (2012). An EST-derived SNP and SSR genetic linkage map of cassava (Manihot esculenta Crantz). Theoretical and Applied Genetics, 125(2), 329-342.
dc.identifier.issn0040-5752
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1412
dc.descriptionPublished online: 15 march, 2012
dc.description.abstractCassava (Manihot esculenta Crantz) is one of the most important food security crops in the tropics and increasingly being adopted for agro-industrial processing. Genetic improvement of cassava can be enhanced through marker-assisted breeding. For this, appropriate genomic tools are required to dissect the genetic architecture of economically important traits. Here, a genome-wide SNPbased genetic map of cassava anchored in SSRs is presented. An outbreeder full-sib (F1) family was genotyped on two independent SNP assay platforms: an array of 1,536 SNPs on Illumina’s GoldenGate platform was used to genotype a first batch of 60 F1. Of the 1,358 successfully converted SNPs, 600 which were polymorphic in at least one of the parents and was subsequently converted to KBiosciences’ KASPar assay platform for genotyping 70 additional F1. High-precision genotyping of 163 informative SSRs using capillary electrophoresis was also carried out. Linkage analysis resulted in a final linkage map of 1,837 centi-Morgans (cM) containing 568 markers (434 SNPs and 134 SSRs) distributed across 19 linkage groups. The average distance between adjacent markers was 3.4 cM. About 94.2% of the mapped SNPs and SSRs have also been localized on scaffolds of version 4.1 assembly of the cassava draft genome sequence. This more saturated genetic linkage map of cassava that combines SSR and SNP markers should find several applications in the improvement of cassava including aligning scaffolds of the cassava genome sequence, genetic analyses of important agro-morphological traits, studying the linkage disequilibrium landscape and comparative genomics.
dc.description.sponsorshipCGIAR Generation Challenge Program
dc.format.extent329–342
dc.language.isoen
dc.subjectCassava
dc.subjectGenetic
dc.subjectFood Security
dc.subjectManihot Esculenta
dc.titleAn ESTderived SNP and SSR genetic linkage map of cassava (Manihot esculenta Crantz)
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUkiriguru Agricultural Research Institute, Tanzania
cg.contributor.affiliationSugarcane Research Institute, Tanzania
cg.contributor.affiliationUniversity of Arizona
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryTanzania
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCassava
cg.iitasubjectGenetic Improvement
cg.journalTheoretical and Applied Genetics
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid79553
cg.identifier.doihttps://dx.doi.org/10.1007/s00122-012-1836-4


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