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dc.contributor.authorRoderick, H.
dc.contributor.authorTripathi, L.
dc.contributor.authorBabirye, A.
dc.contributor.authorWang, D.
dc.contributor.authorTripathi, J.N.
dc.contributor.authorUrwin, P.E.
dc.contributor.authorAtkinson, H.J.
dc.date.accessioned2019-12-04T11:04:23Z
dc.date.available2019-12-04T11:04:23Z
dc.date.issued2012-03
dc.identifier.citationRoderick, H., Tripathi, L., Babirye, A., Wang, D., Tripathi, J., Urwin, P.E. & Atkinson, H.J. (2012). Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes. Molecular Plant Pathology, 13(8), 842-851.
dc.identifier.issn1464-6722
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1416
dc.description.abstractPlant parasitic nematodes impose a severe constraint on plantain and banana productivity; however, the sterile nature of many cultivars precludes conventional breeding for resistance. Transgenic plantain cv. Gonja manjaya (Musa AAB) plants, expressing a maize cystatin that inhibits nematode digestive cysteine proteinases and a synthetic peptide that disrupts nematode chemoreception, were assessed for their ability to resist nematode infection. Lines were generated that expressed each gene singly or both together in a stacked defence. Nematode challenge with a single species or a mixed population identified 10 lines with significant resistance. The best level of resistance achieved against the major pest species Radopholus similis was 84% ± 8% for the cystatin, 66% ± 14% for the peptide and 70% ± 6% for the dual defence. In the mixed population, trial resistance was also demonstrated to Helicotylenchus multicinctus. A fluorescently labelled form of the chemodisruptive peptide underwent retrograde transport along certain sensory dendrites of R. similis as required to disrupt chemoreception. The peptide was degraded after 30 min in simulated intestinal fluid or boiling water and after 1 h in nonsterile soil. In silico sequence analysis suggests that the peptide is not a mammalian antigen. This work establishes the mode of action of a novel nematode defence, develops the evidence for its safe and effective deployment against multiple nematode species and identifies transgenic plantain lines with a high level of resistance for a proposed field trial.
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council, United Kingdom
dc.description.sponsorshipDepartment for International Development, United Kingdom
dc.format.extent842-851
dc.language.isoen
dc.subjectTransgenic
dc.subjectPlantains
dc.subjectResistance
dc.subjectPlant
dc.subjectPathogens
dc.subjectNematodes
dc.titleGeneration of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationUniversity of Leeds
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryUganda
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectPlantain
cg.journalMolecular Plant Pathology
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid79557
cg.identifier.doihttps://dx.doi.org/10.1111/j.1364-3703.2012.00792.x


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