| dc.contributor.author | Eni, A. |
| dc.contributor.author | Hughes, J. |
| dc.contributor.author | Asiedu, Robert |
| dc.contributor.author | Rey, M. |
| dc.date.accessioned | 2019-12-04T11:07:44Z |
| dc.date.available | 2019-12-04T11:07:44Z |
| dc.date.issued | 2012 |
| dc.identifier.citation | Eni, A., Hughes, J., Asiedu, R., & Rey, M. (2012). Re-evaluation of yam mosaic virus (YMV) detection methods. Academic Journal of Plant Sciences, 5(1), 18-22. |
| dc.identifier.issn | 1995-8986 |
| dc.identifier.uri | https://hdl.handle.net/20.500.12478/1551 |
| dc.description.abstract | Accurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), the most commonly used detection method for YMV, detected the virus in significantly less leaf samples than immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) necessitated a re-evaluation of YMV detection methods. In the present study, eighteen previously tested YMV positive leaf samples from Benin and Ghana were re-tested using TAS-ELISA, Protein A-sandwich (PAS) ELISA and IC-RT-PCR. Three sap dilutions, 1/10, 1/50 and 1/100, were tested for each sample. Both at 1/10 and 1/50 dilutions, PAS-ELISA and IC-RT-PCR detected YMV in 11 (61.1%) and 12 (66.7%) of the leaves respectively. Virus detection by PAS-ELISA reduced to 50% at 1/100 sap dilution and increased to 77.8% in IC-RT-PCR. YMV detection by TAS-ELISA varied between 38.9% and 16.7% at 1/10 and 1/100 dilutions respectively. These results indicate a deficiency in the use of TAS-ELISA as a sole YMV certification method since the detecting monoclonal antibody used in this assay may be strain specific. The use of PAS-ELISA at a 1/10 sap dilution is suggested for YMV detection where the facilities for molecular detection are unavailable. |
| dc.language.iso | en |
| dc.subject | Yam Mosaic Virus |
| dc.subject | Detection Sensitivity |
| dc.subject | Genus Potyvirus |
| dc.subject | Yams |
| dc.subject | Dioscorea |
| dc.subject | Epidemiological |
| dc.subject | Isolation |
| dc.subject | Alkaline Phosphatase |
| dc.title | Re-evaluation of Yam Mosaic Virus (YMV) detection methods |
| dc.type | Journal Article |
| dc.description.version | Peer Review |
| cg.contributor.crp | Roots, Tubers and Bananas |
| cg.contributor.affiliation | Covenant University |
| cg.contributor.affiliation | International Institute of Tropical Agriculture |
| cg.contributor.affiliation | University of the Witwatersrand |
| cg.coverage.region | Africa |
| cg.coverage.region | West Africa |
| cg.coverage.country | Benin |
| cg.coverage.country | Ghana |
| cg.authorship.types | CGIAR and developing country institute |
| cg.iitasubject | Yam |
| cg.iitasubject | Plant Diseases |
| cg.journal | Academic Journal of Plant Sciences |
| cg.howpublished | Formally Published |
| cg.accessibilitystatus | Limited Access |
| local.dspaceid | 82112 |
| cg.identifier.doi | https://dx.doi.org/10.5829/idosi.ajps.2012.5.1.10512 |
