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dc.contributor.authorEni, A.
dc.contributor.authorHughes, J.
dc.contributor.authorAsiedu, Robert
dc.contributor.authorRey, M.
dc.date.accessioned2019-12-04T11:07:44Z
dc.date.available2019-12-04T11:07:44Z
dc.date.issued2012
dc.identifier.citationEni, A., Hughes, J., Asiedu, R., & Rey, M. (2012). Re-evaluation of yam mosaic virus (YMV) detection methods. Academic Journal of Plant Sciences, 5(1), 18-22.
dc.identifier.issn1995-8986
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1551
dc.description.abstractAccurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), the most commonly used detection method for YMV, detected the virus in significantly less leaf samples than immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) necessitated a re-evaluation of YMV detection methods. In the present study, eighteen previously tested YMV positive leaf samples from Benin and Ghana were re-tested using TAS-ELISA, Protein A-sandwich (PAS) ELISA and IC-RT-PCR. Three sap dilutions, 1/10, 1/50 and 1/100, were tested for each sample. Both at 1/10 and 1/50 dilutions, PAS-ELISA and IC-RT-PCR detected YMV in 11 (61.1%) and 12 (66.7%) of the leaves respectively. Virus detection by PAS-ELISA reduced to 50% at 1/100 sap dilution and increased to 77.8% in IC-RT-PCR. YMV detection by TAS-ELISA varied between 38.9% and 16.7% at 1/10 and 1/100 dilutions respectively. These results indicate a deficiency in the use of TAS-ELISA as a sole YMV certification method since the detecting monoclonal antibody used in this assay may be strain specific. The use of PAS-ELISA at a 1/10 sap dilution is suggested for YMV detection where the facilities for molecular detection are unavailable.
dc.language.isoen
dc.subjectYam Mosaic Virus
dc.subjectDetection Sensitivity
dc.subjectGenus Potyvirus
dc.subjectYams
dc.subjectDioscorea
dc.subjectEpidemiological
dc.subjectIsolation
dc.subjectAlkaline Phosphatase
dc.titleRe-evaluation of Yam Mosaic Virus (YMV) detection methods
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationCovenant University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of the Witwatersrand
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryBenin
cg.coverage.countryGhana
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectYam
cg.iitasubjectPlant Diseases
cg.journalAcademic Journal of Plant Sciences
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid82112
cg.identifier.doihttps://dx.doi.org/10.5829/idosi.ajps.2012.5.1.10512


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