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dc.contributor.authorOnyilo, F.
dc.contributor.authorTusiime, Geoffrey
dc.contributor.authorChen, L.H.
dc.contributor.authorFalk, B.
dc.contributor.authorStergiopoulos, I.
dc.contributor.authorTripathi, J.N.
dc.contributor.authorTushemereirwe, W.K.
dc.contributor.authorKubiriba, J.
dc.contributor.authorChanga, C.
dc.contributor.authorTripathi, L.
dc.date.accessioned2019-12-04T11:08:36Z
dc.date.available2019-12-04T11:08:36Z
dc.date.issued2017-05-16
dc.identifier.citationOnyilo, F., Tusiime, G., Chen, L.H., Falk, B., Stergiopoulos, I., Tripathi, J.N., ... & Tripathi, L. (2017). Agrobacterium tumefaciens-mediated transformation of Pseudocercospora fijiensis to determine the role of PfHog1 in osmotic stress regulation and virulence modulation. Frontiers in Microbiology, 8, 830.
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1869
dc.descriptionOpen Access Journal; Published online: 16 May 2017
dc.description.abstractBlack Sigatoka disease, caused by Pseudocercospora fijiensis is a serious constraint to banana production worldwide. The disease continues to spread in new ecological niches and there is an urgent need to develop strategies for its control. The high osmolarity glycerol (HOG) pathway in Saccharomyces cerevisiae is well known to respond to changes in external osmolarity. HOG pathway activation leads to phosphorylation, activation and nuclear transduction of the HOG1 mitogen-activated protein kinases (MAPKs). The activated HOG1 triggers several responses to osmotic stress, including up or down regulation of different genes, regulation of protein translation, adjustments to cell cycle progression and synthesis of osmolyte glycerol. This study investigated the role of the MAPK-encoding PfHog1 gene on osmotic stress adaptation and virulence of P. fijiensis. RNA interference-mediated gene silencing of PfHog1 significantly suppressed growth of P. fijiensis on potato dextrose agar media supplemented with 1 M NaCl, indicating that PfHog1 regulates osmotic stress. In addition, virulence of the PfHog1-silenced mutants of P. fijiensis on banana was significantly reduced, as observed from the low rates of necrosis and disease development on the infected leaves. Staining with lacto phenol cotton blue further confirmed the impaired mycelial growth of the PfHog1 in the infected leaf tissues, which was further confirmed with quantification of the fungal biomass using absolute- quantitative PCR. Collectively, these findings demonstrate that PfHog1 plays a critical role in osmotic stress regulation and virulence of P. fijiensis on its host banana. Thus, PfHog1 could be an interesting target for the control of black Sigatoka disease in banana.
dc.description.sponsorshipUnited States Agency for International Development
dc.description.sponsorshipNorman Borlaug Leadership Enhancement in Agriculture Program
dc.format.extent1-13
dc.language.isoen
dc.subjectAgrobacterium Tumefaciens
dc.subjectTransformation
dc.subjectOsmotic Stress
dc.subjectVirulence
dc.subjectPseudocercospora Fijiensis
dc.subjectHog1
dc.titleAgrobacterium tumefaciens-mediated transformation of Pseudocercospora fijiensis to determine the role of PfHog1 in osmotic stress regulation and virulence modulation
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationNational Agricultural Research Organisation, Uganda
cg.contributor.affiliationMakerere University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of California
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.journalFrontiers in Microbiology
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid83622
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.3389/fmicb.2017.00830


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