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Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory

dc.contributor.authorKihika, R.
dc.contributor.authorMurungi, L.K.
dc.contributor.authorCoyne, D.L.
dc.contributor.authorNg'ang'a, M.
dc.contributor.authorHassanali, A.
dc.contributor.authorTeal, P.E.A.
dc.contributor.authorTorto, B.
dc.date.accessioned2019-12-04T11:08:41Z
dc.date.available2019-12-04T11:08:41Z
dc.date.issued2017
dc.identifier.citationKihika, R., Murungi, L.K., Coyne, D., Hassanali, A., Teal, P.E. & Torto, B. (2017). Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory. Scientific Reports, 7(1), 2903.
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1889
dc.descriptionOpen Access Journal; Published online: 06 June 2017
dc.description.abstractPlant volatile signatures are often used as cues by herbivores to locate their preferred hosts. Here, we report on the volatile organic compounds used by the subterranean root-knot nematode (RKN) Meloidogyne incognita for host location. We compared responses of infective second stage juveniles (J2s) to root volatiles of three cultivars and one accession of the solanaceous plant, Capsicum annum against moist sand in dual choice assays. J2s were more attracted to the three cultivars than to the accession, relative to controls. GC/MS analysis of the volatiles identified common constituents in each plant, five of which were identified as α-pinene, limonene, 2-methoxy-3-(1-methylpropyl)-pyrazine, methyl salicylate and tridecane. We additionally identified thymol as being specific to the accession. In dose-response assays, a blend of the five components elicited positive chemotaxis (71–88%), whereas individual components elicited varying responses; Methyl salicylate (MeSA) elicited the highest positive chemotaxis (70–80%), α-pinene, limonene and tridecane were intermediate (54–60%), and 2-methoxy- 3-(1-methylpropyl)-pyrazine the lowest (49–55%). In contrast, thymol alone or thymol combined with either the preferred natural plant root volatiles or the five-component synthetic blend induced negative chemotaxis. Our results provide insights into RKN-host plant interactions, creating new opportunities for plant breeding programmes towards management of RKNs.
dc.description.sponsorshipUnited States Department of Agriculture
dc.description.sponsorshipDepartment for International Development, United Kingdom
dc.description.sponsorshipSwedish International Development Cooperation Agency
dc.description.sponsorshipSwiss Agency for Development and Cooperation
dc.description.sponsorshipGovernment of Kenya
dc.format.extent1-10
dc.language.isoen
dc.subjectNematode
dc.subjectMeloidogyne Incognita
dc.subjectLimonene
dc.subjectRoot-Knot Nematode
dc.subjectCultivars
dc.subjectCapsicum Annum
dc.subjectRoot Herbivory
dc.subjectRoot Volatiles
dc.subjectPlant Hosts
dc.titleParasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Centre of Insect Physiology and Ecology
cg.contributor.affiliationJomo Kenyatta University of Agriculture and Technology
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationKenyatta University
cg.contributor.affiliationUnited States Department of Agriculture
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPests Of Plants
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Ecology
cg.journalScientific Reports
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid83909
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1038/s41598-017-02379-8


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