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First report of maize yellow mosaic virus infecting sugarcane (Saccharum spp.) and itch grass (Rottboellia cochinchinensis) in Nigeria

dc.contributor.authorYahaya, A.
dc.contributor.authorAl Rwahnih, M.
dc.contributor.authorDangora, D.B.
dc.contributor.authorGregg, L.
dc.contributor.authorAlegbejo, M.D.
dc.contributor.authorKumar, P.L.
dc.contributor.authorAlabi, O.J.
dc.date.accessioned2019-12-04T11:08:42Z
dc.date.available2019-12-04T11:08:42Z
dc.date.issued2017-07-09
dc.identifier.citationYahaya, A., Al Rwahnih, M., Dangora, D.B., Gregg, L., Alegbejo, M.D., Kumar, P.L. & Alabi, O.J. (2017). First report of maize yellow mosaic virus infecting sugarcane (Saccharum spp.) and itch grass (Rottboellia cochinchinensis) in Nigeria. Plant Disease,101(7), 1335.
dc.identifier.issn0191-2917
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1892
dc.descriptionArticle purchased; Published online: 9 May 2017
dc.description.abstractDuring routine surveys conducted from February to July 2015 in the northern guinea savannah region of Nigeria, sugarcane and itch grass (Rottboellia cochinchinensis) plants showing virus-like mosaic symptoms were encountered in farmers’ sugarcane fields in Kaduna State. Symptomatic leaf tissue samples from five randomly selected plants (sugarcane = 4; itch grass = 1) were dried and stored under CaCl2 at room temperature then shipped to Texas A&M AgriLife Research and Extension Center, Weslaco, TX, with USDA-APHIS-PPQ permit (P526P-14-04321) for further analysis. The MagMAX-96 viral RNA isolation kit (Thermo Fisher) was used to isolate total nucleic acid (TNA) from each sample and from a sample subset consisting of pooled leaf tissue materials from both plants. TNA aliquot from the pooled sample was subjected to ribosomal RNA depletion and cDNA library construction using a TruSeq Stranded Total RNA with Ribo-Zero Plant kit (Illumina), then sequenced on the Illumina NextSeq 500 platform. The raw high-throughput sequencing (HTS) reads were analyzed as previously described (Alabi et al. 2015), generating ∼43.5 million Illumina reads (76 nucleotides [nt] in length), of which 31,486 de novo assembled
dc.format.extent1-2
dc.language.isoen
dc.subjectMaize
dc.subjectDiseases
dc.subjectDna
dc.subjectSugarcane
dc.subjectMosaic Virus
dc.subjectItch Grass
dc.titleFirst report of maize yellow mosaic virus infecting sugarcane (Saccharum spp.) and itch grass (Rottboellia cochinchinensis) in Nigeria
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationAhmadu Bello University
cg.contributor.affiliationUniversity of California
cg.contributor.affiliationTexas A&M University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectMaize
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Genetic Resources
cg.journalPlant Disease
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid83942
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1094/pdis-03-17-0315-pdn


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