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Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease

dc.contributor.authorNamukwaya, B.
dc.contributor.authorTripathi, L.
dc.contributor.authorTripathi, J.N.
dc.contributor.authorArinaitwe, G.
dc.contributor.authorMukasa, S.B.
dc.contributor.authorTushemereirwe, W.K.
dc.date.accessioned2019-12-04T11:10:14Z
dc.date.available2019-12-04T11:10:14Z
dc.date.issued2012-08
dc.identifier.citationNamukwaya, B., Tripathi, L., Tripathi, J.N., Arinaitwe, G., Mukasa, S.B. & Tushemereirwe, W.K. (2012). Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease. Transgenic Research, 21(4), 855-865.
dc.identifier.issn0962-8819
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2034
dc.descriptionPublished online: 19 November 2011
dc.description.abstractBanana Xanthomonas wilt (BXW), caused by Xanthomonas campestris pv. musacearum, is one of the most important diseases of banana (Musa sp.) and currently considered as the biggest threat to banana production in Great Lakes region of East and Central Africa. The pathogen is highly contagious and its spread has endangered the livelihood of millions of farmers who rely on banana for food and income. The development of disease resistant banana cultivars remains a high priority since farmers are reluctant to employ labor-intensive disease control measures and there is no host plant resistance among banana cultivars. In this study, we demonstrate that BXW can be efficiently controlled using transgenic technology. Transgenic bananas expressing the plant ferredoxin-like protein (Pflp) gene under the regulation of the constitutive CaMV35S promoter were generated using embryogenic cell suspensions of banana. These transgenic lines were characterized by molecular analysis. After challenge with X.campestris pv. musacearum transgenic lines showed high resistance. About 67% of transgenic lines evaluated were completely resistant to BXW. These transgenic lines did not show any disease symptoms after artificial inoculation of in vitro plants under laboratory conditions as well as potted plants in the screen-house, whereas non-transgenic control plants showed severe symptoms resulting in complete wilting. This study confirms that expression of the Pflp gene in banana results in enhanced resistance to BXW. This transgenic technology can provide a timely solution to the BXW pandemic.
dc.description.sponsorshipGatsby Charitable Foundation
dc.description.sponsorshipAfrican Agricultural Technology Foundation
dc.format.extent855-865
dc.language.isoen
dc.subjectBananas
dc.subjectCultivars
dc.subjectDisease Resistance
dc.subjectTransgenic Banana
dc.subjectBanana Xanthomonas Wilt
dc.subjectPflp Gene
dc.titleTransgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationNational Agricultural Research Organisation, Uganda
cg.contributor.affiliationMakerere University
cg.coverage.regionAfrica
cg.coverage.regionCentral Africa
cg.coverage.regionEast Africa
cg.coverage.countryBurundi
cg.coverage.countryCongo, Dr
cg.coverage.countryEthiopia
cg.coverage.countryKenya
cg.coverage.countryRwanda
cg.coverage.countryTanzania
cg.coverage.countryUganda
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectBanana
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Genetic Resources
cg.journalTransgenic Research
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid85876
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1007/s11248-011-9574-y


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