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    Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease

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    U17ArtJinBioinformaticsInthomNodev.pdf (706.7Kb)
    Date
    2017-11
    Author
    Jin, Y.
    Goodman, R.E.
    Tetteh, A.O.
    Lu, M.
    Tripathi, L.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Banana Xanthomonas wilt (BXW) disease threatens banana production and food security throughout East Africa. Natural resistance is lacking among common cultivars. Genetically modified (GM) bananas resistant to BXW disease were developed by inserting the hypersensitive response-assisting protein (Hrap) or/and the plant ferredoxin-like protein (Pflp) gene(s) from sweet pepper (Capsicum annuum). Several of these GM banana events showed 100% resistance to BXW disease under field conditions in Uganda. The current study evaluated the potential allergenicity and toxicity of the expressed proteins HRAP and PFLP based on evaluation of published information on the history of safe use of the natural source of the proteins as well as established bioinformatics sequence comparison methods to known allergens (www.AllergenOnline.org and NCBI Protein) and toxins (NCBI Protein). The results did not identify potential risks of allergy and toxicity to either HRAP or PFLP proteins expressed in the GM bananas that might suggest potential health risks to humans. We recognize that additional tests including stability of these proteins in pepsin assay, nutrient analysis and possibly an acute rodent toxicity assay may be required by national regulatory authorities.
    http://dx.doi.org/10.1016/j.fct.2017.08.024
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2241
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1016/j.fct.2017.08.024
    IITA Subjects
    Banana; Disease Control; Food Security; Plant Genetic Resources
    Agrovoc Terms
    Bananas; Allergen; Toxin; Risk Assessment; Hrap; Pflp; Genetically Modified Banana; Banana Xanthomonas Wilt; Bioinformatics
    Regions
    Africa; Central Africa; East Africa
    Countries
    Burundi; Congo, Dr; Kenya; Tanzania; Uganda
    Journals
    Food and Chemical Toxicology
    Collections
    • Journal and Journal Articles5283
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