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    A time series transcriptome analysis of cassava (Manihot esculenta Crantz) varieties challenged with Ugandan cassava brown streak virus

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    U17ArtAmugeTimeInthomDev.pdf (3.572Mb)
    Date
    2017
    Author
    Amuge, T.
    Berger, D.K.
    Katari, M.S.
    Myburg, A.A.
    Goldman, S.L.
    Ferguson, M.E.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    A time-course transcriptome analysis of two cassava varieties that are either resistant or susceptible to cassava brown streak disease (CBSD) was conducted using RNASeq, after graft inoculation with Ugandan cassava brown streak virus (UCBSV). From approximately 1.92 billion short reads, the largest number of differentially expressed genes (DEGs) was obtained in the resistant (Namikonga) variety at 2 days after grafting (dag) (3887 DEGs) and 5 dag (4911 DEGs). At the same time points, several defense response genes (encoding LRR-containing, NBARC-containing, pathogenesis-related, late embryogenesis abundant, selected transcription factors, chaperones, and heat shock proteins) were highly expressed in Namikonga. Also, defense-related GO terms of ‘translational elongation’, ‘translation factor activity’, ‘ribosomal subunit’ and ‘phosphorelay signal transduction’, were overrepresented in Namikonga at these time points. More reads corresponding to UCBSV sequences were recovered from the susceptible variety (Albert) (733 and 1660 read counts per million (cpm)) at 45 dag and 54 dag compared to Namikonga (10 and 117 cpm respectively). These findings suggest that Namikonga’s resistance involves restriction of multiplication of UCBSV within the host. These findings can be used with other sources of evidence to identify candidate genes and biomarkers that would contribute substantially to knowledge-based resistance breeding.
    http://dx.doi.org/10.1038/s41598-017-09617-z
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2390
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1038/s41598-017-09617-z
    IITA Subjects
    Cassava
    Agrovoc Terms
    Cassava; Diseases; Disease Control; Namikonga
    Regions
    Africa; East Africa
    Countries
    Uganda
    Journals
    Scientific Reports
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    • Journal and Journal Articles5283
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