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    Impaired expressions of the beta and delta isoforms of vacuolar processing enzymes compromise the basal defenses of Arabidopsis thaliana against the phloem-feeding insect Myzus persicae

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    S17ArtAlpuertoImpairedInthomNodev.pdf (1.136Mb)
    Date
    2017-10
    Author
    Alpuerto, J.B.
    Mukherjee, A.
    Kitazumi, A.
    Alyokhin, A.
    Koeyer, D. de
    Reyes, B.G. de los
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    While the functions of vacuolar processing enzymes (VPE) in plant responses to pathogens are now better established, their possible roles against insect herbivores need validation. Inspired by an observation that βVPE and δVPE represent the most highly upregulated transcripts in leaves of Solanum bulbocastanum and its backcross progenies that were highly resistant to the phloem-feeding green peach aphids (Myzus persicae), we used a reverse genetic approach in Arabidopsis to investigate whether T-DNA insertion mutants for specific βVPE and δVPE loci would exhibit compromised defenses at the whole-plant, physiological, and molecular levels. We hypothesized that impaired expression of VPE genes would lead to hyper-susceptibility if VPE-mediated processes were essential for basal defenses, given that Arabidopsis is naturally susceptible. Compared to the wild-type, βvpe and δvpe mutants exhibited an early onset of leaf curling and necrosis, rapid aphid population build-up, and significant physiological tissue injuries as a result of herbivory. This indicates that basal defenses were compromised by the loss-of-functions of either VPE-encoding gene. VPE co-expression networks were comprised of genes involved in sucrose metabolism and transport (AtSUC5, AtSUS3, and invertases), efflux and oxidative detoxification proteins, and modulators of gene-for-gene-type disease response linked to sucrose metabolism (AtSWEET13 and AtSWEET15). These results suggest that VPEs play a role in basal defenses by mediating mechanisms of detoxification and metabolite availability in host tissues to counteract the rapid depletion caused by the phloem sucker. These processes might be important as first line of defense to delay the progression of perturbations caused by herbivory.
    http://dx.doi.org/10.1007/s11738-017-2529-z
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2395
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1007/s11738-017-2529-z
    IITA Subjects
    Genetic Improvement; Pests Of Plants; Plant Genetic Resources
    Agrovoc Terms
    Reactive Oxygen Species; Detoxification; Sucrose; Metabolism; Oxidative; Basal Defenses; Vacuole-Mediated Programmed Cell Death; Oxidative-Mediated Network
    Regions
    South America
    Countries
    Colombia
    Journals
    Acta Physiologiae Plantarum
    Collections
    • Journal and Journal Articles5283
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