• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Regional Heritability Mapping provides insights into Dry matter (DM) Content in African white and yellow cassava populations

    Thumbnail
    View/Open
    U17ArtOkekeRegionalInthomNodev.pdf (1.096Mb)
    Date
    2017
    Author
    Okeke, U.G.
    Akdemir, D.
    Rabbi, Ismail Y
    Kulakow, P.A.
    Jannink, Jean-Luc
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    The HarvestPlus program for cassava (Manihot esculenta Crantz) fortifies cassava with beta-carotene by breeding for carotene-rich tubers (yellow cassava). However, a negative correlation between yellowness and dry matter (DM) content has been identified. Here, we investigated the genetic control of DM in white and yellow cassava subpopulations. We used regional heritability mapping (RHM) to associate DM to genomic segments in both subpopulations. Significant segments were subjected to candidate gene analysis and we attempted to validate candidates using prediction accuracies. The RHM procedure was validated using a simulation approach. The RHM revealed significant hits for white cassava on chromosomes 1, 4, 5, 10, 17 and 18 while hits for the yellow were on chromosome 1. Candidate gene analysis revealed genes in the carbohydrate biosynthesis pathway including the plant serine-threonine protein kinases (SnRKs), UDP-glycosyltransferases, UDP-sugar transporters, invertases, pectinases, and regulons. Validation using 1252 unique identifiers from the SnRK gene family genome-wide recovered 50% of the predictive accuracy of whole genome SNPs for DM while validation using 53 likely (extracted from literature) genes from significant segments recovered 32%. Genes including an acid invertase, a neutral/alkaline invertase and a glucose-6-phosphate isomerase were validated based on an a priori list for the cassava starch pathway and also a fructose-biphosphate aldolase from the calvin cycle pathway. The power of the RHM procedure was estimated at 47 percent when the causal QTL generated 10% of the phenotypic variance with sample size of 451. Cassava DM genetics is complex. RHM may be useful for complex traits.
    http://dx.doi.org/10.3835/plantgenome2017.06.0050
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2404
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.3835/plantgenome2017.06.0050
    IITA Subjects
    Cassava; Genetic Improvement; Plant Genetic Resources
    Agrovoc Terms
    Cassava; Gene; Cassava Starch; Regional Heritability Mapping; Dry Matter Content; Beta-Carotene; Yellow Cassava; White Cassava
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    Plant Genome
    Collections
    • Journal and Journal Articles4835

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      Cassava production and processing in the Democratic Republic of Congo: COSCA working paper, No 22 

      Nweke, F.; Lutete, D.; Dixon, A.; Ugwu, B.; Ajobo, O.; Kalombo, N.; Bukaka, B. (2000)
      The importance of cassava relative to other crops in the cropping system was almost total; cassava was present in 70% of arable fields; maize and beans or peas were each present in 13% of arable fields and all other crops were present in 4%. Cassava was produced mostly for sale, yet it was not as important around market centers as in remote areas because imported rice and wheat products were easily accessible. Cassava land area was not expanding in many of the villages visited because of low-level ...
    • Thumbnail

      Molecular biodiversity of cassava begomoviruses in Tanzania: evolution of cassava geminiviruses in Africa and evidence for East Africa being a center of diversity of cassava geminiviruses 

      Ndunguru, J.; Legg, J.P.; Aveling, T.; Thompson, G.; Fauquet, Claude M. (2005)
      Cassava is infected by numerous geminiviruses in Africa and India that cause devastating losses to poor farmers. We here describe the molecular diversity of seven representative cassava mosaic geminiviruses (CMGs) infecting cassava from multiple locations in Tanzania. We report for the first time the presence of two isolates in East Africa: (EACMCV-[TZ1] and EACMCV-[TZ7]) of the species East African cassava mosaic Cameroon virus, originally described in West Africa. The complete nucleotide sequence ...
    • Thumbnail

      Recently released cassava varieties in the lake zone of Tanzania 

      Mkamilo, G.S.; Ndyetabula, I.L.; Jeremiah, S.C.; Merumba, S.M.; Kibura, J.; Karugaba, R.K; Chirimi, B.; Bagambisa, C.; Milala, P.; Chuwa, C.; Ngendello, T.; Legg, J.P.; Kanju, E.E.; Ntawuruhunga, Pheneas (2010)
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository