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    Midgut bacterial diversity analysis of laboratory reared and wild Anopheles gambiae and Culex quinquefasciatus mosquitoes in Kenya

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    U17ArtMwadondoMidgutNothomDev.pdf (649.6Kb)
    Date
    2017-08
    Author
    Mwadondo, E.M.
    Ghilamicael, A.
    Alakonya, A.E.
    Kasili, R.W.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Midgut symbiotic bacteria are known to play fundamental roles in the biology of mosquitoes, however knowledge of midgut bacterial communities associated with mosquitoes is scanty due to limitation of the isolation techniques based on culturing. In this study, the composition and diversity of midgut bacteria in field collected and lab reared adult female Anopheles gambiae and Culex quinquefasciatus mosquitoes was explored using the Illumina sequencing. Deoxyribonucleic acid was isolated from the pooled midgut extracts and their 16S rRNA gene sequenced using Illumina sequencing platform. Operational taxonomic units (OTUs) were analyzed using QIIME 1.8.0; taxonomy was assigned using BLASTn against SILVA 119 and hierarchical clustering was done using R program software. Out of the total number of sequence reads obtained, 145 OTUs were realized at 3% genetic distance. The 145 OTUs spanned 12 phyla; Proteobacteria, Firmicutes, Cyanobacteria, Euryarchaeota, Gemmatimonadetes, Spirochaetae, Archeabacteria Verrucomicrobia, Chloroflexi, Bacteriodetes, Acidobacteria and Actinobacteria. Microbial community composition based on OTUs showed significant difference between field collected and lab reared mosquitoes (ᵪ2 = 45.0799, p = 3.2 × 10-5). Similarly, there was a significant difference in community composition at OTU level between Anopheles gambiae and Culex quinquefasciatus (ᵪ2 = 31.2257, p = 7.7 × 10-4). The bacterial composition and diversity appeared to be influenced by the environment and the species of the mosquitoes.
    http://dx.doi.org/10.5897/ajmr2016.8256
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2427
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.5897/ajmr2016.8256
    IITA Subjects
    Disease Control
    Agrovoc Terms
    Anopheles Gambiae; Culex Quinquefasciatus; Dna; Mosquitoes; Midgut; Diversity
    Regions
    Africa; West Africa
    Countries
    Kenya
    Journals
    African Journal of Microbiology Research
    Collections
    • Journal and Journal Articles4835
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