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Rapid analyses of dry matter content and carotenoids in fresh cassava roots using a portable visible and near infrared spectrometer (Vis/NIRS)

dc.contributor.authorIkeogu, Ugochukwu N.
dc.contributor.authorDavrieux, Fabrice
dc.contributor.authorDufour, D.L.
dc.contributor.authorCeballos, H.
dc.contributor.authorEgesi, Chiedozie N.
dc.contributor.authorJannink, Jean-Luc
dc.date.accessioned2019-12-04T11:11:27Z
dc.date.available2019-12-04T11:11:27Z
dc.date.issued2017-12-11
dc.identifier.citationIkeogu, Ugochukwu N.; Davrieux, Fabrice; Dufour, Dominique; Ceballos, Hernan; Egesi, Chiedozie N.; Jannink, Jean-Luc. 2017. Rapid analyses of dry matter content and carotenoids in fresh cassava roots using a portable visible and near infrared spectrometer (Vis/NIRS) . PLoS ONE 12(12): e0188918.
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2432
dc.description.abstractPortable Vis/NIRS are flexible tools for fast and unbiased analyses of constituents with minimal sample preparation. This study developed calibration models for dry matter content (DMC) and carotenoids in fresh cassava roots using a portable Vis/NIRS system. We examined the effects of eight data pre-treatment combinations on calibration models and assessed calibrations on processed and intact root samples. We compared Vis/NIRS derived-DMC to other phenotyping methods. The results of the study showed that the combination of standard normal variate and de-trend (SNVD) with first derivative calculated on two data points and no smoothing (SNVD+1111) was adequate for a robust model. Calibration performance was higher with processed than the intact root samples for all the traits although intact root models for some traits especially total carotenoid content (TCC) (R2c = 96%, R2cv = 90%, RPD = 3.6 and SECV = 0.63) were sufficient for screening purposes. Using three key quality traits as templates, we developed models with processed fresh root samples. Robust calibrations were established for DMC (R2c = 99%, R2cv = 95%, RPD = 4.5 and SECV = 0.9), TCC (R2c = 99%, R2cv = 91%, RPD = 3.5 and SECV = 2.1) and all Trans β-carotene (ATBC) (R2c = 98%, R2cv = 91%, RPD = 3.5 and SECV = 1.6). Coefficient of determination on independent validation set (R2p) for these traits were also satisfactory for ATBC (91%), TCC (88%) and DMC (80%). Compared to other methods, Vis/NIRS-derived DMC from both intact and processed roots had very high correlation (>0.95) with the ideal oven-drying than from specific gravity method (0.49). There was equally a high correlation (0.94) between the intact and processed Vis/NIRS DMC. Therefore, the portable Vis/NIRS could be employed for the rapid analyses of DMC and quantification of carotenoids in cassava for nutritional and breeding purposes.
dc.format.extent12(12): e0188918
dc.language.isoen
dc.publisherPublic Library of Science
dc.subjectCassava
dc.subjectManihot Esculenta
dc.subjectCarotenoids
dc.subjectInfrared Spectrophotometry
dc.subjectSpecific Gravity
dc.subjectHigh Performance Liquid Chromatography
dc.subjectCloning
dc.subjectPhenotypes
dc.subjectCarotenoides
dc.subjectEspectroscopia Infrarroja
dc.subjectPeso Específico
dc.subjectCromatografía Líquida De Alta Resolución
dc.subjectCromatografía Líquida Alta Presión
dc.subjectClonación
dc.titleRapid analyses of dry matter content and carotenoids in fresh cassava roots using a portable visible and near infrared spectrometer (Vis/NIRS)
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationCornell University
cg.contributor.affiliationNational Root Crops Research Institute, Nigeria
cg.contributor.affiliationCentre de Coopération Internationale en Recherche Agronomique pour le Développement
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationInternational Center for Tropical Agriculture
cg.contributor.affiliationUnited States Department of Agriculture
cg.creator.identifierDominique Dufour: 0000-0002-6046-0741
cg.creator.identifierHernan Ceballos: 0000-0002-8744-7918
cg.isijournalISI Journal
cg.journalPLoS ONE
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid92994
cg.identifier.doihttps://doi.org/10.1371/journal.pone.0188918


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