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    Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions

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    beiseigel-iron-2007.pdf (242.4Kb)
    Date
    2007
    Author
    Beiseigel, J.M.
    Hunt, J.R.
    Glahn, Raymond P.
    Welch, R.M.
    Menkir, A.
    Maziya-Dixon, B.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Background: An in vitro digestion and Caco-2 cell model may predict iron bioavailability to humans; however, direct comparisons are lacking. Objective: The objective was to test the differences in iron bioavailability between 2 maize varieties and 2 bean varieties (white beans and colored beans) by comparing human, Caco-2, and algorithm results. Design: Two randomized, 2 × 2 factorial experiments compared women's iron absorption from 2 maize varieties (ACR and TZB; n = 26) and 2 bean varieties (great northern and pinto; n = 13), each fed with and without ascorbic acid (AA) from orange juice. Nonheme iron bioavailability was determined from 2-wk retention of extrinsic radioiron tracers and was compared with Caco-2 cell and algorithm results from identical meals. Results: Without AA supplementation, women absorbed only about 2% of the iron from the maize or bean meals. The results were unaffected by the variety of either maize or beans. Adding AA (15–20 molar ratios of AA:iron) roughly tripled the iron absorption (P < 0.0001) from all test meals. Although the Caco-2 model predicted a slightly improved bioavailability of iron from ACR maize than from TZB maize (P < 0.05), it accurately predicted relative iron absorption from the maize meals. However, the Caco-2 model inaccurately predicted both a considerable difference between bean varieties (P < 0.0001) and a strong interaction between bean varieties and enhancement by AA (P < 0.0001). The algorithm method was more qualitatively than quantitatively useful and requires further development to accurately predict the influence of polyphenols on iron absorption. Conclusions: Caco-2 predictions confirmed human iron absorption results for maize meals but not for bean meals, and algorithm predictions were only qualitatively predictive.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2451
    Non-IITA Authors ORCID
    Abebe Menkirhttps://orcid.org/0000-0002-5907-9177
    IITA Subjects
    Maize
    Agrovoc Terms
    Bioavailability; Ascorbic Acid; Phytic Acid; Polyphenols; Tannins; Beans; Maize
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    American Journal of Clinical Nutrition
    Collections
    • Journal and Journal Articles5283
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