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Identifying aflatoxin resistance related proteins/genes through proteomics and RNAi gene silencing

dc.contributor.authorChen, Z.Y.
dc.contributor.authorBrown, R.L.
dc.contributor.authorGuo, B.Z.
dc.contributor.authorMenkir, A.
dc.contributor.authorCleveland, T.E.
dc.date.accessioned2019-12-04T11:11:36Z
dc.date.available2019-12-04T11:11:36Z
dc.date.issued2009
dc.identifier.citationChen, Z.Y., Brown, R.L., Guo, B.Z., Menkir, A. & Cleveland, T.E. (2009). Identifying aflatoxin resistance-related proteins/genes through proteomics and RNAi gene silencing. Peanut Science, 36(1), 35-41.
dc.identifier.issn0095-3679
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2464
dc.description.abstractAflatoxins are carcinogenic secondary metab-olites produced mainly byAspergillus flavusLinkex. Fries, andA. prarasiticusSpeare duringinfection of susceptible crops, such as maize,cottonseed, peanuts and tree nuts. This paper willreview research efforts in identifying aflatoxinresistance-related proteins/genes in maize. Similarstrategies may be useful in peanut. For maize,although genotypes resistant toA. flavusinfectionor aflatoxin production have been identified, theincorporation of resistance into commercial lineshas been slow due to the lack of selectable markersand poor understanding of host resistance mech-anisms. Recently, resistance-associated proteins(RAPs) were identified through proteomic com-parison of constitutive protein profiles betweenresistant and susceptible maize genotypes. Theseproteins belong to three major groups based ontheir peptide sequence homologies: storage pro-teins, stress-related proteins, and antifungal pro-teins. Preliminary characterization of some ofthese RAPs suggest that they play a direct role inhost resistance, such as pathogenesis-relatedprotein 10 (PR10), or an indirect role, such asglyoxalase I (GLX I), through enhancing the hoststress tolerance. To verify whether these RAPsplay a role in host resistance, RNA interference(RNAi) gene silencing technique was used tosilence the expression of these genes in maize.RNAi vectors (glx IRNAi andpr10RNAi) wereconstructed using Gateway technology, and thentransformed into immature maize embryos usingboth bombardment andAgrobacteriuminfection.The extent of gene silencing in transgenic callustissues ranged from 20% to over 99%. The RNAisilenced transgenic maize seeds have also beenobtained from plants regenerated fromAgrobac-teriumtransformed callus lines. Kernel screenassay of the transgenic maize kernels demonstrat-ed a significant increase in susceptibility toA.flavuscolonization and aflatoxin production insome of the silenced transgenic lines comparedwith non-silenced control kernels, suggesting thedirect involvement of these two proteins inaflatoxin resistance in maize.
dc.description.sponsorshipUnited States Department of Agriculture
dc.format.extent35-41
dc.language.isoen
dc.publisherPeanut Science
dc.subjectMaize
dc.subjectKernel Protein
dc.subjectResistance Mechanisms
dc.subjectAflatoxins
dc.subjectGenotypes
dc.subjectDrought
dc.subjectPeanut
dc.subjectGermplasm
dc.subjectPathogen
dc.titleIdentifying aflatoxin resistance related proteins/genes through proteomics and RNAi gene silencing
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationLouisiana State University
cg.contributor.affiliationUnited States Department of Agriculture
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAcp
cg.coverage.regionAfrica
cg.coverage.regionNorth America
cg.coverage.regionWest Africa
cg.coverage.countryUnited States
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAflatoxin
cg.iitasubjectCrop Husbandry
cg.iitasubjectDisease Control
cg.iitasubjectFood Science
cg.iitasubjectGenetic Improvement
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.iitasubjectNutrition
cg.iitasubjectPlant Genetic Resources
cg.journalPeanut Science
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid93215
cg.identifier.doihttps://dx.doi.org/10.3146/AT07-005-1


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