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Serological properties of ordinary and necrotic isolates of Potato virus Y: a case study of PVYN misidentification

dc.contributor.authorKarasev, A.V.
dc.contributor.authorNikolaeva, O.V.
dc.contributor.authorHu, X.
dc.contributor.authorSielaff, Z.
dc.contributor.authorWhitworth, J.
dc.contributor.authorLorenzen, J.H.
dc.contributor.authorGray, S.M.
dc.date.accessioned2019-12-04T11:11:41Z
dc.date.available2019-12-04T11:11:41Z
dc.date.issued2009
dc.identifier.citationKarasev, A.V., Nikolaeva, O.V., Hu, X., Sielaff, Z., Whitworth, J., Lorenzen, J.H. & Gray, S.M. (2009). Serological properties of ordinary and necrotic isolates of Potato virus Y: a case study of PVYN misidentification. American Journal of Potato Research, 87(1), 1-9.
dc.identifier.issn1099-209X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2479
dc.description.abstractIn the course of a multi-year survey of Potato virus Y (PVY) incidence and diversity in the U.S. seed potato crop, an unusual PVY variant was identified in low but significant levels in multiple states. This variant, PVYO-O5, was initially detected by a commercially available PVYN-specific monoclonal antibody, 1F5. This antibody is widely used by U.S. Seed Certification programs to test for PVYN and is one of two antibodies designated by the North American Plant Protection Organization (NAPPO) for pre-shipment testing of tuber lots that are to be transported between countries. Consequently, PVYN positives identified by the 1F5 antibody have triggered quarantine actions, prevented cross-border shipments and impacted trade. Here, we demonstrate by a variety of methods that the PVYO-O5 is a variant within the ordinary PVY strain (PVYO). Specifically, the PVYO-O5 variant likely arose due to a single amino acid substitution within the capsid protein. This variant does not induce vein necrosis in tobacco or tuber necrosis in susceptible varieties of potato. Furthermore, it is identified by RT-PCR based diagnostics as PVYO and it has a typical PVYO genome sequence. We demonstrate that another PVYN specific monoclonal antibody, SASA-N, recognizes an epitope distinct from that recognized by 1F5, and correctly identifies the PVYO-O5 variants as belonging to the PVYO serotype. Since the PVYO-O5 variant is present in many seed producing states and misidentification of PVYO-O5 as PVYN/NTN has clear quarantine implications for export shipments of potato, the limitations of the commercially available monoclonal antibodies should be considered in any certification or phytosanitary testing program
dc.description.sponsorshipNational Potato Council
dc.description.sponsorshipIdaho Potato Commission
dc.description.sponsorshipUnited States Department of Agriculture
dc.format.extent1-9.
dc.language.isoen
dc.subjectAntibodies
dc.subjectPotato
dc.subjectPotato Virus Y
dc.subjectPotato Production
dc.titleSerological properties of ordinary and necrotic isolates of Potato virus Y: a case study of PVYN misidentification
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationUniversity of Idaho
cg.contributor.affiliationUnited States Department of Agriculture
cg.contributor.affiliationCornell University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAcp
cg.coverage.regionNorth America
cg.coverage.countryUnited States
cg.coverage.countryCanada
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectPlant Diseases
cg.iitasubjectPests Of Plants
cg.iitasubjectDisease Control
cg.iitasubjectSoil Information
cg.iitasubjectSoil Surveys And Mapping
cg.journalAmerican Journal of Potato Research
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid93230
cg.identifier.doihttps://dx.doi.org/10.1007/s12230-009-9110-2


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