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Review of immunological methods for the quantification of aflatoxins in peanut and other foods

dc.contributor.authorWaliyar, F.
dc.contributor.authorReddy, S.V.
dc.contributor.authorKumar, P.L.
dc.date.accessioned2019-12-04T11:11:43Z
dc.date.available2019-12-04T11:11:43Z
dc.date.issued2009
dc.identifier.citationWaliyar, F., Reddy, S.V. & Kumar, P.L. (2009). Review of immunological methods for the quantification of aflatoxins in peanut and other foods. Peanut Science, 36(1), 54-59.
dc.identifier.issn0095-3679
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2485
dc.description.abstractAflatoxin contamination is widespread instaple crops like peanut, maize, sorghum, pearlmillet, chillies, pistachio, cassava etc., and com-promises the safety of food and feed supplies. It isimportant to be able to detect and quantifyaflatoxins in commodities to protect human andanimal health. Many different methods, includingantibody-based ones, are available for quantita-tive estimation of aflatoxins. However, most ofthese methods such as HPLC, HPTLC, and TLCare expensive and/or difficult to use in developedcountries. Using the state-of-the-art facilities atICRISAT, we developed polyclonal and mono-clonal antibodies for the detection of totalaflatoxins, aflatoxin B1 and M1 (secreted in milk).These were used to develop a simple andinexpensive competitive enzyme-linked immuno-sorbent assays (cELISA) that has lower detectionlimits (1.0mg/kg) and cost (about $1 per sample)less than other available methods. More than 100samples can be analyzed in a day. These tests haveprovided a unique opportunity for ICRISAT andits partners to conduct field studies to selectresistant genotypes, identify high risk populationsand determine the dietary sources to stimulateappropriate interventions to enhance the food andhuman health safety, trade and thereby farmers’income
dc.format.extent54-59
dc.language.isoen
dc.subjectPeanut
dc.subjectArachis Hypogaea
dc.subjectCompetitive Elisa
dc.subjectImmunological Methods
dc.subjectAflatoxins
dc.subjectGenotypes
dc.subjectChromatography
dc.subjectPolyclonal
dc.titleReview of immunological methods for the quantification of aflatoxins in peanut and other foods
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Crops Research Institute for the Semi-Arid Tropics
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionAcp
cg.coverage.regionWest Africa
cg.coverage.regionEast Asia
cg.coverage.countryNiger
cg.coverage.countryIndia
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAflatoxin
cg.iitasubjectCrop Husbandry
cg.iitasubjectCrop Systems
cg.iitasubjectDisease Control
cg.iitasubjectFarm Management
cg.iitasubjectFood Security
cg.iitasubjectGenetic Improvement
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.iitasubjectLivelihoods
cg.journalPeanut Science
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid93236


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