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First report of banana bunchy top virus in banana and plantain (Musa spp.) in Angola

dc.contributor.authorKumar, P.L.
dc.contributor.authorAyodele, M.
dc.contributor.authorOben, T.T.
dc.contributor.authorMahungu, N.M.
dc.contributor.authorBeed, Fen D.
dc.contributor.authorCoyne, D.L.
dc.contributor.authorLonda, L.
dc.contributor.authorMutunda, M.P.
dc.contributor.authorKiala, D.
dc.contributor.authorMaruthi, M.N.
dc.date.accessioned2019-12-04T11:11:49Z
dc.date.available2019-12-04T11:11:49Z
dc.date.issued2009
dc.identifier.citationKumar, P.L., Ayodele, M., Oben, T.T., Mahungu, N.M., Beed, F., Coyne, D. & Maruthi, M.N. (2009). First report of banana bunchy top virus in banana and plantain (Musa spp.) in Angola. Plant Pathology, 58, 402-402.
dc.identifier.issn0032-0862
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2503
dc.descriptionFirst published: 11 March 2009
dc.description.abstractIn April 2008 in the northern Provinces of Angola, banana (AAA genome)and plantain (AAB genome) plants (Musa spp.) with severe stunting andmoderate to severe leaf symptoms typical of banana bunchy top diseasewere observed to be widespread in farmers’ fields in Kirimadhola villagein the Ndalatando region in Cuanza Norte Province, and Tokosiala, Bukuli Tsiela and Conde villages in the Landana region of Cabinda Province.Leaf extracts from affected plants were positive in an ELISA assay(OD405nm of infected ≥ 2 × healthy controls) using a polyclonal antiserum(NCBR, India) raised against Banana bunchy top virus (BBTV; genusBabuvirus, family Nanoviridae). BBTV was further confirmed by PCRusing total DNA extracted from plant samples with symptoms andoligonucleotide primers specific for a ca. 250 bp conserved domain ofBBTV DNA-R, according to published PCR protocols (Mansoor et al.,2005). An amplicon of the expected size (256 bp) was cloned into pGEM-T Easy Vector (Promega) and two independent clones were sequencedin both directions (GenBank Acc. No. EU851977). Comparisons ofthis sequence using a blast search showed the greatest nucleotideidentity (97%) with a BBTV isolate from Hawaii (U18077) and 96 to 97%similarity with BBTV isolates of a ‘South Pacific Group’. By contrast,there was less similarity (85 to 90%) with isolates of the ‘Asian Group’,indicating that the virus isolate from Angola falls within the South PacificGroup.This is the first report of BBTV occurrence in Angola. Together with pre-vious reports of BBTV in southern and central African countries (Kenyonet al., 1997; CABI, 2005), this report provides evidence for the widespreadoccurrence of BBTV in the region. The presence of both the virus and itsaphid vector, Pentalonia nigronervosa, in Africa has the potential to causewidespread damage to banana, especially on the highly susceptible Caven-dish types. This highlights the urgent need for intensive surveys to assessthe extent of the geographic spread and severity of BBTV in Africa and theimplementation of phytosanitary measures to eradicate infected plants.
dc.format.extent402-402
dc.language.isoen
dc.subjectAaa Genome
dc.subjectDna
dc.subjectBanana Bunchy Top Disease
dc.subjectPentalonia Nigronervosa
dc.subjectPlantains
dc.titleFirst report of banana bunchy top virus in banana and plantain (Musa spp.) in Angola
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationNatural Resources Institute, Finland
cg.coverage.regionAcp
cg.coverage.regionAfrica
cg.coverage.regionEurope
cg.coverage.regionWest Africa
cg.coverage.regionSouthern Africa
cg.coverage.regionEast Africa
cg.coverage.countryNigeria
cg.coverage.countryMalawi
cg.coverage.countryUganda
cg.coverage.countryAngola
cg.coverage.countryFinland
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlantain
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectDisease Control
cg.iitasubjectFarm Management
cg.iitasubjectBanana
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.journalPlant Pathology
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid93254
cg.identifier.doihttps://dx.doi.org/10.1111/j.1365-3059.2008.01974.x


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