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dc.contributor.authorOdipio, J.
dc.contributor.authorTusiime, Geoffrey
dc.contributor.authorTripathi, L.
dc.contributor.authorAritua, Valentine
dc.date.accessioned2019-12-04T11:11:55Z
dc.date.available2019-12-04T11:11:55Z
dc.date.issued2009
dc.identifier.citationOdipio, J., Tusiime, G., Tripathi, L. & Aritua, V. (2009). Genetic homogeneity among Ugandan isolates of Xanthomonas campestris pv. musacearum revealed by randomly amplified polymorphic DNA analysis. African Journal of Biotechnology, 8(21), 5652-5660.
dc.identifier.issn1684-5315
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2522
dc.descriptionOpen Access Journal
dc.description.abstractThe Random Amplification of Polymorphic DNA (RAPD) analysis was used to detect the genetic diversity among Ugandan isolates of Xanthomonas campestris pv. musacearum (Xcm), the causal agent of banana Xanthomonas wilt (BXW) disease. Seven random primers were used because of their ability to amplify reproducible and reliable fingerprints generated between 6 - 12 amplicons each from the Xcm isolates obtained from central core of pseudostems, peduncles, fruit peelings, sap, nectar,insects’ bodies and bacterial oozes. Regardless of the source and geographical origin, similar fingerprints were generated from the tested isolates. Using a similarity coefficient of 58%, the unweighted pair group method with arithmetic averaging (UPGMA) analysis did not reveal any significant differences in clustering, with exception of a single isolate that had unique fingerprints. Prior to the genetic analysis, all the isolates compared showed no significant difference (P = 0.92) with regard to incubation period for appearance of symptoms and the severity of symptoms in pathogenicity test. Thus, our data indicates that the population of Xcm in Uganda is clonal, that is, one uniform population being spread fast and efficiently, suggesting that there is a low likelihood of the current population to rapidly evolve, in the near future, into more virulent strains to overcome any resistance deployed.
dc.format.extent5652-5660
dc.language.isoen
dc.subjectBanana Xanthomonaswilt
dc.subjectDna Fingerprints
dc.subjectGenetic Variation
dc.subjectXanthomonas Campestris Pv. Musacearum
dc.titleGenetic homogeneity among Ugandan isolates of Xanthomonas campestris pv. musacearum revealed by randomly amplified polymorphic DNA analysis
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationMakerere University
cg.contributor.affiliationNational Agricultural Research Organisation, Uganda
cg.contributor.affiliationKansas State University
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryUganda
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Diseases
cg.iitasubjectDisease Control
cg.iitasubjectGenetic Improvement
cg.journalAfrican Journal of Biotechnology
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid93273


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