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dc.contributor.authorGedil, M.
dc.contributor.authorKolade, F.
dc.contributor.authorRaji, A.
dc.contributor.authorIngelbrecht, I.L.
dc.contributor.authorDixon, A.
dc.date.accessioned2019-12-04T11:13:53Z
dc.date.available2019-12-04T11:13:53Z
dc.date.issued2009
dc.identifier.citationGedil, M., Kolade, F., Raji, A., Ingelbrecht, I. & Dixon, A. (2009). Development of molecular genomic tools for verification of intergeneric hybrids between castor bean (Ricinus communis L.) and cassava (Manihot esculenta Crantz). Journal of Food, Agriculture & Environment, 7(2), 534-539.
dc.identifier.issn1459-0255
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2530
dc.description.abstractDespite its relatively recent introduction to Africa, cassava (Manihot esculenta Crantz) has become a major food crop adapted to a wide range of agro-ecologies that is grown by millions of small-holding farmers of sub-Saharan Africa (SSA), owing to its remarkable tolerance to an array of biotic and abiotic stresses. Its economic importance is expected to grow further as evidenced by global consideration of cassava as an alternative source of renewable energy (biofuel) that prompted genome sequencing initiative of the crop. To address the evolving demand for new cultivars with unique traits such as high quality and quantity starch, high protein and high carotene content, breeders seek to broaden the genetic base of the breeding pool. Secondly, there is a dire need to develop alternative mapping populations such as doubled haploid (DH) to enhance the efficiency of molecular breeding. Towards these goals, we launched a project to cross cassava (Manihot esculenta) with castor bean (Ricinus communis L.). As a first step of validation of hybridization, different types of molecular markers (RAPD, AFLP, and SSR) were screened to distinguish cassava, castor and the putative hybrids at the DNA level. Only few of the RAPD and SSR primers screened showed that some hybrids might have combined alleles from both parents. Some of the primers revealed a castor-specific band that was absent in both cassava and the hybrid. In AFLP analysis, some primer combinations detected both castor and cassava specific bands in the hybrid. However, these data were not consistent with most hybrids and results are inconclusive. In the latest approach, we used bioinformatics tools to design a castor specific sequence tagged site (STS) primer. Preliminary results show that castor-specific STS primer pairs could not amplify products in the putative hybrids while cassava- specific primers amplify the products. Comparison of relative nuclear DNA content with flow cytometry showed that hybrids have similar DNA content with cassava suggesting that the tested genotypes may not be true hybrids. Further crossing and testing of hybrids, including reciprocals and a panel of cassava and castor clones will continue. The development of markers to verify such wide crosses, once confirmed by cytological analysis and morphological characterization, opens an opportunity to use DH mapping populations thereby accelerating the development of saturated linkage map in cassava, with the possibility of introgressing novel traits of commercial value from castor to cassava.
dc.format.extent534-539
dc.language.isoen
dc.publisherScience and Technology
dc.subjectCassava
dc.subjectManihot Esculenta
dc.subjectCastor Bean
dc.subjectRicinus Communis
dc.subjectFlow Cytometry
dc.subjectPloidy Analysis
dc.subjectWide Cross
dc.subjectHybridization
dc.subjectMarker
dc.subjectDoubled Haploid
dc.subjectIntrogression
dc.subjectHybridization
dc.subjectDna
dc.subjectHybrid
dc.subjectMapping Population
dc.titleDevelopment of molecular genomic tools for verification of intergeneric hybrids between castor bean (Ricinus communis L.) and cassava (Manihot esculenta Crantz)
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR single centre
cg.iitasubjectCassava
cg.iitasubjectFarming Systems
cg.iitasubjectFood Security
cg.iitasubjectImpact Assessment
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Production
cg.iitasubjectValue Chains
cg.iitasubjectPests Of Plants
cg.journalJournal of Food, Agriculture & Environment
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid93281


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