Show simple item record

Mixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo

dc.contributor.authorBirindwa, B.A.
dc.contributor.authorGitao, C.G.
dc.contributor.authorBisimwa, P.N.
dc.contributor.authorOkafor, C.
dc.contributor.authorBebora, C.L.
dc.date.accessioned2019-12-04T11:14:14Z
dc.date.available2019-12-04T11:14:14Z
dc.date.issued2017-12
dc.identifier.citationBirindwa, B.A., Gitao, C.G., Bisimwa, P.N., Okafor, C. & Bebora, C.L. (2017). Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo. Journal of Advanced Veterinary and Animal Research, 4(4), 348-355.
dc.identifier.issn2311-7710
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2700
dc.descriptionOpen Access Journal; Published online: 21 Dec 2017
dc.description.abstractObjective: We aimed at determining the prevalence and characterizing the CaPV, determining the CaPV-PPRV coinfection prevalence and providing data about phylogenetic relationship between the fusion protein of PPRV and P32 gene of CaPV. Materials and methods: A total of 150 samples including animals swabs, tissues and blood were collected from unvaccinated goats in a PPR and/or Capripox outbreaks in South Kivu, Eastern of Democratic Republic of the Congo. Conventional PCR and reverse transcriptase (RT-PCR) were used respectively to amplify P32, RPO30, GPCR genes of Capripox virus and Fusion (F) protein of PPRV. Positive samples were sequenced for phylogenetic analysis. Results: Out of 150 tested animals, 64.7% (n=97/150) were PPRV positive, 52.7% (n=79/150) were Capripox positive and 38.7% (n=58/150) were positive for both PPRV and CaPV. The pairwise comparison of P32gene of CaPV and F gene of PPRV showed 99.75% of identity percentage among goatpox virus sequences, 96.95% among PPRV sequences and 47.91% between CaPV and PPRV sequences. Conclusion: The study has demonstrated high prevalence of CaP V-PPRV mixed infection in South Kivu. Lumpy skinvirus disease (LSVD) is a lineage circulating which has a genetic relationship between its P32gene and the Fgene of PPRV giving the challenge to differentiate the two diseases at the clinical farm level.
dc.format.extent348-355
dc.language.isoen
dc.subjectCapripox
dc.subjectCapripoxvirus
dc.subjectDemocratic Republic Of The Congo
dc.subjectGoat
dc.subjectPhylogeny
dc.subjectCoinfection
dc.titleMixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationUniversité Evangélique en Afrique
cg.contributor.affiliationUniversity of Nairobi
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionCentral Africa
cg.coverage.countryCongo, Dr
cg.creator.identifierChris Okafor: 0000-0003-2162-6101
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectBiodiversity
cg.journalJournal of Advanced Veterinary and Animal Research
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid93624
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.5455/javar.2017.d233


Files in this item

Thumbnail
Name:
U17ArtBirindwaMixedNothomDev.pdf
Size:
909.3Kb
Format:
PDF
View/Open

This item appears in the following Collection(s)

Show simple item record