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    Homeolog expression analysis in an allotriploid non-model crop via integration of transcriptomics and proteomics

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    U18ArtWesemaelHomeologInthomNodev.pdf (1.980Mb)
    Date
    2018
    Author
    Wesemael, J. van
    Hueber, Y.
    Kissel, E.
    Campos, N.
    Swennen, R.L.
    Carpentier, S.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The fate of doubled genes, from allopolyploid or autopolyploid origin, is controlled at multiple levels, resulting in the modern day cultivars. We studied the root growth of 3 different triploid banana cultivars under control and osmotic stress conditions. The root growth of the allopolyploid ABB cultivar was 42% higher under control and 61% higher under osmotic stress. By integrating transcriptomics and proteomics, we studied the gene expression of all 3 cultivars, resulting in 2,749 identified root proteins. 383 gene loci displayed genotype specific differential expression whereof 252 showed at least one Single Amino Acid Polymorphism (SAAP). In the ABB cultivar, allele expressions supposedly follow a 1/3 and 2/3 pattern for respectively the A and the B allele. Using transcriptome read alignment to assess the homeoallelic contribution we found that 63% of the allele specific genes deviated from this expectation. 32 gene loci even did not express the A allele. The identified ABB allele- specific proteins correlate well with the observed growth phenotype as they are enriched in energy related functions such as ATP metabolic processes, nicotinamide nucleotide metabolic processes, and glycolysis.
    http://dx.doi.org/10.1038/s41598-018-19684-5
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2711
    Non-IITA Authors ORCID
    Rony Swennenhttps://orcid.org/0000-0002-5258-9043
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1038/s41598-018-19684-5
    Research Themes
    BIOTECH & PLANT BREEDING
    IITA Subjects
    Banana; Genetic Improvement; Plant Breeding; Plant Genetic Resources; Plant Production; Plantain
    Agrovoc Terms
    Bananas; Plantains; Cultivars; Nicotinamide; Musa; Genotypes; Allopolyploid; Autopolyploid; Transcriptomics; Proteomics
    Regions
    Acp; Europe
    Countries
    Belgium
    Journals
    Scientific Reports
    Collections
    • Journal and Journal Articles5078
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