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dc.contributor.authorSilva, G.
dc.contributor.authorOyekanmi, J.
dc.contributor.authorNkere, C.K.
dc.contributor.authorBömer, M.
dc.contributor.authorKumar, P.L.
dc.contributor.authorSeal, S.E.
dc.date.accessioned2019-12-04T11:14:25Z
dc.date.available2019-12-04T11:14:25Z
dc.date.issued2018-04-01
dc.identifier.citationSilva, G., Oyekanmi, J., Nkere, C.K., Bömer, M., Kumar, P.L. & Seal, S.E. (2018). Rapid detection of potyviruses from crude plant extracts. Analytical Biochemistry, 546, 17-22.
dc.identifier.issn0003-2697
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2757
dc.descriptionArticle purchased; Published online: 31 Jan 2018
dc.description.abstractPotyviruses (genus Potyvirus; family Potyviridae) are widely distributed and represent one of the most economically important genera of plant viruses. Therefore, their accurate detection is a key factor in developing efficient control strategies. However, this can sometimes be problematic particularly in plant species containing high amounts of polysaccharides and polyphenols such as yam (Dioscorea spp.). Here, we report the development of a reliable, rapid and cost-effective detection method for the two most important potyviruses infecting yam based on reverse transcription-recombinase polymerase amplification (RT-RPA). The developed method, named ‘Direct RT-RPA’, detects each target virus directly from plant leaf extracts prepared with a simple and inexpensive extraction method avoiding laborious extraction of high-quality RNA. Direct RT-RPA enables the detection of virus-positive samples in under 30 min at a single low operation temperature (37 °C) without the need for any expensive instrumentation. The Direct RT-RPA tests constitute robust, accurate, sensitive and quick methods for detection of potyviruses from recalcitrant plant species. The minimal sample preparation requirements and the possibility of storing RPA reagents without cold chain storage, allow Direct RT-RPA to be adopted in minimally equipped laboratories and with potential use in plant clinic laboratories and seed certification facilities worldwide.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent17-22
dc.language.isoen
dc.subjectYams
dc.subjectPotyvirus
dc.subjectPlant Viruses
dc.subjectFood Security
dc.subjectRecombinase Polymerase Amplification
dc.subjectIsothermal Amplification
dc.subjectRapid Diagnostics
dc.subjectCrude Plant Extracts
dc.titleRapid detection of potyviruses from crude plant extracts
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpMaize
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationUniversity of Greenwich
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationNational Root Crops Research Institute, Nigeria
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryCote D'Ivoire
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPests Of Plants
cg.iitasubjectYam
cg.journalAnalytical Biochemistry
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid93789
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1016/j.ab.2018.01.019


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