| dc.contributor.author | Narina, S.S. |
| dc.contributor.author | Mohammed, I. |
| dc.contributor.author | Asiedu, Robert |
| dc.contributor.author | Mignouna, H.D. |
| dc.date.accessioned | 2019-12-04T11:14:28Z |
| dc.date.available | 2019-12-04T11:14:28Z |
| dc.date.issued | 2009 |
| dc.identifier.citation | Narina, S.S., Mohamed, A.I., Asiedu, R. & Mignouna, H.D. (2009). A Standardized RNA Isolation Protocol for Yam (Dioscorea alata L) cDNA Library Construction. Virginia Journal of Science, 60(4),171-180. |
| dc.identifier.issn | 0042-658X |
| dc.identifier.uri | https://hdl.handle.net/20.500.12478/2771 |
| dc.description.abstract | For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate University. The RNA isolated using this standardized protocol was high in quality and led to successful good quality cDNA library construction and identification of functional ESTs in yam. |
| dc.description.sponsorship | United States Agency for International Development |
| dc.language.iso | en |
| dc.subject | Yams |
| dc.subject | Genotypes |
| dc.subject | Colletotrichum Gloeosporioides |
| dc.subject | Cdna |
| dc.subject | Rna |
| dc.subject | Diseases |
| dc.title | A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction |
| dc.type | Journal Article |
| dc.description.version | Peer Review |
| cg.contributor.affiliation | Virginia State University |
| cg.contributor.affiliation | United States Department of Agriculture |
| cg.contributor.affiliation | International Institute of Tropical Agriculture |
| cg.contributor.affiliation | African Agricultural Technology Foundation |
| cg.coverage.region | Africa |
| cg.coverage.region | West Africa |
| cg.coverage.country | Nigeria |
| cg.isijournal | ISI Journal |
| cg.authorship.types | CGIAR and developing country institute |
| cg.iitasubject | Yam |
| cg.iitasubject | Disease Control |
| cg.iitasubject | Genetic Improvement |
| cg.iitasubject | Plant Diseases |
| cg.accessibilitystatus | Limited Access |
| local.dspaceid | 93872 |
