| dc.contributor.author | Bhattacharjee, R. |
| dc.contributor.author | Ferguson, M. |
| dc.contributor.author | Gedil, M. |
| dc.contributor.author | Dumet, D. |
| dc.contributor.author | Ingelbrecht, I.L. |
| dc.date.accessioned | 2019-12-04T11:14:43Z |
| dc.date.available | 2019-12-04T11:14:43Z |
| dc.date.issued | 2009 |
| dc.identifier.citation | Bhattacharjee, R., Ferguson, M., Gedil, M., Dumet, D. & Ingelbrecht, I. (2009). Field collection, preservation and large scale DNA extraction procedures for cassava (Manihot esculenta Crantz.). African Journal of Biotechnology, 8(15), p. 3424-3430. |
| dc.identifier.issn | 1684-5315 |
| dc.identifier.uri | https://hdl.handle.net/20.500.12478/2839 |
| dc.description.abstract | Some genetic studies using molecular methods such as diversity assessment or marker-assisted selection require collection of a large number of samples from fields located in the vicinity or in remote areas, followed by isolation of good quality DNA in a short time span. In the present study, different tissue preservation methods were compared for subsequent DNA extraction using a modified CTAB method in two 96-well plates, following grinding of leaf tissues with a GenoGrinder 2000. We found that preservation of leaf tissues in NaCl-CTAB-azide buffer (as described in Rogstad, 1992) at 4°C is a better storage procedure than preservation at -20°C to obtain good quality DNA. Comparison of DNA extraction with or without use of phenol revealed that the quality of DNA was not drastically affected when non-phenol extraction protocol was used and did not affect PCR amplification. Thus, the recommended DNA extraction procedure allowed us to process 192 samples per day at a cost of $0.80 per sample, with an average yield of 1.8 g, suitable for both PCR and genotyping. |
| dc.description.sponsorship | Global Crop Diversity Trust |
| dc.language.iso | en |
| dc.subject | Cassava |
| dc.subject | Nacl-Ctab-Azide Solution |
| dc.subject | Phenol |
| dc.subject | Genogrinder 2000 |
| dc.title | Field collection,preservation and large scale DNA extraction procedures for cassava (Manihot esculenta Crantz.) |
| dc.type | Journal Article |
| dc.description.version | Peer Review |
| cg.contributor.affiliation | International Institute of Tropical Agriculture |
| cg.coverage.region | Africa |
| cg.coverage.region | West Africa |
| cg.coverage.region | East Africa |
| cg.coverage.country | Nigeria |
| cg.coverage.country | Kenya |
| cg.authorship.types | CGIAR single centre |
| cg.iitasubject | Cassava |
| cg.iitasubject | Genetic Improvement |
| cg.iitasubject | Tissue Culture |
| cg.iitasubject | Markets |
| cg.accessibilitystatus | Open Access |
| local.dspaceid | 93940 |
