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    Genetic diversity in wild relatives of cowpea (Vigna unguiculata) as revealed by simple sequence repeats (SSR) markers

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    Date
    2008
    Author
    Ogunkanmi, L.A.
    Ogundipe, O.T.
    Ng, N.Q.
    Fatokun, C.A.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    The genetic diversity in wild cowpea was assessed using cowpea derived simple sequence repeat (SSR) markers. A total of forty eight wild cowpea lines collected from diverse geographical locations in Africa were used for the study. Twelve SSR markers were selected for amplification of DNA of the test lines and these produced a total of ninety polymorphic bands. A dendrogram constructed using unweighted pair group using mathematical averages (UPGMA) analysis distinguished forty three accessions out of a total of forty eight accessions used for the study implying that a great diversity in wild cowpea. Accessions from Southern part of Africa have the highest polymorphism information content (PIC) values suggesting a high level of diversity from that region and is likely to be center of diversity of wild cowpea. The allele number per primer varied from 4 to 13 with an average of 7.5 alleles. The SSR, VM 36 showed the highest number of alleles with the PIC value of 0.870. This analysis revealed that SSR markers are useful tools in assessing the level of genetic diversity in wild cowpea.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2853
    IITA Subjects
    Grain Legumes; Cowpea; Agribusiness; Crop Systems; Disease Control; Farm Management; Biodiversity; Food Security; Forestry; Handling, Transport, Storage And Protection Of Agricultural Products; Domestic Trade
    Agrovoc Terms
    Wild Cowpea; Vigna Unguiculata; Genetic Variation; Microsatellites; Ssrs; Pic; Alleles
    Regions
    Africa; Asia; West Africa; Southeast Asia
    Countries
    Nigeria; Thailand
    Collections
    • Journal and Journal Articles4835
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