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Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants

dc.contributor.authorYusuf, M.
dc.contributor.authorRaji, A.A.
dc.contributor.authorIngelbrecht, I.L.
dc.contributor.authorKatung, M.D.
dc.date.accessioned2019-12-04T11:15:09Z
dc.date.available2019-12-04T11:15:09Z
dc.date.issued2008
dc.identifier.citationYusuf, M., Raji, A.A., Ingelbrecht, I. & Katung, M.D. (2008). Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants. African Journal of Plant Science, 2(9), 105-108.
dc.identifier.issn1996 0824
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2925
dc.descriptionOpen Access Journal
dc.description.abstractThe experiment was conducted to test the in vitro regeneration efficiency of cowpea shoots via embryonic axes explants and the effect of benzyl amino purine (BAP) concentration on shoot formation. A total of 600 explants (embryonic axes) were plated from which 237 shoots were regenerated. The average regeneration ratio was 39% and the number of shoot per explant obtained stood at an average of 6.0. This indicates likelihood that higher regeneration percent and number of shoots per explant could be possible as more proficiency is attained in the use of the protocol. The role of the growth regulator, benzyl amino purine (BAP), as a shoot growth inducing factor and modifier in cowpea in vitro regeneration was demonstrated in the experiment. The protocol used in the experiment gave promising results for the efficient regeneration and subsequent genetic transformation of cowpea.
dc.description.sponsorshipUnited States Agency for International Development
dc.language.isoen
dc.subjectCowpeas
dc.subjectIn Vitro
dc.subjectRegeneration Ratio
dc.subjectBenzyl Amino Purine (Bap)
dc.subjectEmbryonic Axes Explants
dc.subjectGenetic Transformation Of Cowpea
dc.subjectBiotechnology
dc.titleRegeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationAhmadu Bello University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Production
cg.iitasubjectCowpea
cg.iitasubjectCrop Husbandry
cg.iitasubjectPests Of Plants
cg.iitasubjectFood Security
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.iitasubjectLivelihoods
cg.iitasubjectDisease Control
cg.iitasubjectNutrition
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Diseases
cg.accessibilitystatusOpen Access
local.dspaceid94026


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