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    Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L.

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    adeniyi-shoot-2008.pdf (108.2Kb)
    Date
    2008-04
    Author
    Adeniyi, O.J.
    Adetimirin, V.O.
    Ingelbrecht, I.L.
    Asiedu, Robert
    Type
    Journal Article
    Metadata
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    Abstract/Description
    In vitro culture media capable of regenerating moderate to high shoots and/or plantlets from meristems of two yam species – Dioscorea rotundata and Dioscorea alata within comparable duration of 10 weeks as commonly obtained in other monocots and root and tuber crops were investigated. The study comprised 125 phytohormone combinations investigated in three factorial experiments each consisting of an auxin (NAA) and a cytokinin (BAP or kinetin), or two cytokinins only. The frequency of direct plantlet regeneration, though significantly (P < 0.05) higher for D. alata than for D. rotundata, was low and ranged from 0 to 10% at 3 weeks after culture (WAC) and 0 to 35% at 8 WAC. At 8 WAC, shoot regeneration of 42-75% was obtained in D. rotundata in MS medium supplemented with 0.1 M NAA + 0.20 M BAP, and shoot + plantlet regeneration of 60-82% obtained in media containing 0.05 M + 0.20 M BAP or 0.46 M BAP + 0.50 M kinetin in D. alata. Both shoot induction and plantlet regeneration were species-dependent. Induced shoots were successfully rooted in MS medium within 3 to 4 weeks, bringing time taken for plantlet regeneration to 11 to 12 weeks. Regenerants were morphologically similar to the mother plants. Results of the present study will facilitate regeneration of plantlets via meristem in D. rotundata and D. alata.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2939
    IITA Subjects
    Plant Production; Food Security; Farm Management; Yam; Plant Diseases; Plant Health; Disease Control; Post-Harvesting Technology; Genetic Improvement; Pests Of Plants; Plant Breeding; Plant Genetic Resources
    Agrovoc Terms
    Meristems; Phytohormone Combinations; Plantlets; Shoots; Yams; Dioscorea Rotundata; Yam Mosaic Virus; Germplasm
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Collections
    • Journal and Journal Articles4835
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