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Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virusderived transgenes from plant tissues

dc.contributor.authorNdunguru, J.
dc.contributor.authorTaylor, N.
dc.contributor.authorYadav, J.S.
dc.contributor.authorAly, H.
dc.contributor.authorLegg, J.P.
dc.contributor.authorAveling, T.
dc.contributor.authorThompson, G.
dc.contributor.authorFauquet, Claude M.
dc.date.accessioned2019-12-04T11:18:22Z
dc.date.available2019-12-04T11:18:22Z
dc.date.issued2005
dc.identifier.citationNdunguru, J., Taylor, N., Yadav, J.S., Aly, H., Legg, J., Aveling, T. & Fauquet, C. (2005). Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived trans genes from plant tissues. Virology Journal, 2(45), 1-12.
dc.identifier.issn1743-422X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3182
dc.description.abstractBackground: Plant viral diseases present major constraints to crop production. Effective sampling of the viruses infecting plants is required to facilitate their molecular study and is essential for the development of crop protection and improvement programs. Retaining integrity of viral pathogens within sampled plant tissues is often a limiting factor in this process, most especially when sample sizes are large and when operating in developing counties and regions remote from laboratory facilities. FTA is a paper-based system designed to fix and store nucleic acids directly from fresh tissues pressed into the treated paper. We report here the use of FTA as an effective technology for sampling and retrieval of DNA and RNA viruses from plant tissues and their subsequent molecular analysis. Results: DNA and RNA viruses were successfully recovered from leaf tissues of maize, cassava, tomato and tobacco pressed into FTA® Classic Cards. Viral nucleic acids eluted from FTA cards were found to be suitable for diagnostic molecular analysis by PCR-based techniques andrestriction analysis, and for cloning and nucleotide sequencing in a manner equivalent to that offered by tradition isolation methods. Efficacy of the technology was demonstrated both from sampled greenhouse-grown plants and from leaf presses taken from crop plants growing in farmer's fields in East Africa. In addition, FTA technology was shown to be suitable for recovery of viral- derived transgene sequences integrated into the plant genome. Conclusion: Results demonstrate that FTA is a practical, economical and sensitive method for sampling, storage and retrieval of viral pathogens and plant genomic sequences, when working under controlled conditions and in the field. Application of this technology has the potential to significantly increase ability to bring modern analytical techniques to bear on the viral pathogens infecting crop plants.
dc.language.isoen
dc.subjectMolecular
dc.subjectDna And Rna Viruses
dc.subjectViral Pathogens
dc.subjectCrop Plants
dc.subjectGenomes
dc.subjectGermplasm
dc.titleApplication of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virusderived transgenes from plant tissues
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationDonald Danforth Plant Science Center
cg.contributor.affiliationMinistry of Agriculture and Food Security, Tanzania
cg.contributor.affiliationCairo University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationAgricultural Research Council, South Africa
cg.coverage.regionAcp
cg.coverage.regionAfrica
cg.coverage.regionNorth America
cg.coverage.regionMiddle East
cg.coverage.regionEast Africa
cg.coverage.regionSouthern Africa
cg.coverage.countryUnited States
cg.coverage.countryTanzania
cg.coverage.countryEgypt
cg.coverage.countryUganda
cg.coverage.countrySouth Africa
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectPests Of Plants
cg.iitasubjectPlant Breeding
cg.iitasubjectDiseases Control
cg.iitasubjectFarm Management
cg.iitasubjectFood Security
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Production
cg.accessibilitystatusOpen Access
local.dspaceid94648
cg.identifier.doihttps://dx.doi.org/10.1186/1743-422X-2-45


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